StressMarq/HSP70 Protein/SPR-103C/2x100-µg,StressMarq,,StressMarq,StressMarq Biosciences,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：StressMarq/HSP70 Protein/SPR-103C/2x100-µg

产品编号：SPR-103C

市场价：¥12340.00

场地：美国(厂家直采)

产品分类：生化试剂>缓冲液>酸碱缓冲液>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$617.00

品牌： StressMarq

公司：StressMarq

公司分类：

商品介绍

Overview:

ProductName HSP70Protein

Description

ActiveHumanRecombinantHSP70Protein

Applications WB,SDS-PAGE,ATPaseActivityAssay,FunctionalAssay,ELISA

Concentration Lot/batchspecific.Seeincludeddatasheet.

Conjugates

Histag

Properties

StorageBuffer	Na-Phosphate,pH7.5(20mM),150mMNaCl,10%glycerol,200mMImidazole
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	AffinityPurified
Specificity	~70kDa
CiteThisProduct	HumanRecombinantHSP70Protein(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SPR-103)
CertificateofAnalysis	Thisproducthasbeencertified>90%pureusingSDS-PAGEanalysis.TheproteinhasATPaseactivityatthetimeofmanufactureof3.3µMphosphateliberated/hr/µgproteinina200µlreactionat37°C(pH7.5)inthepresenceof20ulof1mMATPusingaMalachiteGreenassay.

BIOLOGicalDescription

AlternativeNames	HSP701Protein,HSP702Protein,HSP70.1Protein,HSP72Protein,HSP73Protein,HSPA1Protein,HSPA1AProtein,HSPA1BProtein
ResearchAreas	Cancer,HeatShock
CellularLocalization	Cytoplasm
AccessionNumber	NM_005345
GeneID	3303
SwissProt	P08107
ScientificBackground	HSP70genesencodeabundantheat-inducIBLe70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast50%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com.
References	1.ZhoJ.(1998)Cell.94:471-480. 2.Boorstein,W.R.,Ziegelhoffer,T.&Craig,E.A.(1993)J.Mol.Evol.38(1):1-17. 3.RothmanJ.(1989)Cell.59:591-601. 4.DeLuca-Flahertyetal.(1990)Cell.62:875-887. 5.BorkP.,SanderC.&ValenciaA.(1992)Proc.NatlAcad.Sci.USA.89:7290-7294. 6.FinkA.L.(1999)Physiol.Rev.79:425-449. 7.SmithD.F.,etal.,(1993)Mol.Cell.Biol.13(2):869-876. 8.PrapapanichV.,etal.,(1996)Mol.Cell.Biol.16(11):6200-6207. 9.Fernandez-Funezetal.,(2000)Nature.408(6808):101-106.

ProductImages

SDS-PAGEof70kDaHsp70protein(SPR-103).

ProductCitations(7)

ELISA

MeasuringHsp72(HSPA1A)byIndirectSandwichELISA.

Ireland,H.E.andWilliams,J.H.H.(2011)MethodsMolBiol.787,145-153.

PubMedID:21898234Applications:ELISA

TheDangerSignalS100BIntegratesPathogen–andDanger–SensingPathwaystoRestrainInflammation.

Sorci,G.etal.(2011)PLoSPathog.7(3):e1001315.

PubMedID:21423669Applications:ELISA

OtherCitations

Re-examiningHSPC1inhibitors.

Lee,S.L.etal.(2017)CellStressChaperones.22(2):293-306.

PubMedID:28255900Applications:WesternBlotControl

Hsp70PromotesEpithelialSodiumChannelFunctionalExpressionbyIncreasingItsAssociationwithCoatComplexIIandItsExitfromEndoplasmicReticulum.

Chanoux,R.A.etal.(2012)JBiolChem.287.19255-19265.

PubMedID:22496374Applications:WesternBlotControl

IdentificationofthemajorallergenofMalasseziaglobosarelevantforatopicdermatitis.

Ishibashi,Y.,Kato,H.,Asahi,Y.,Suqita,T.andNishikawa,A.(2009)JDermatolSci.55(3):185-192.

PubMedID:19540092Applications:WesternBlotInhibitor

InVivoGenerationofNeurotoxicPrionProtein:RoleforHsp70inAccumulationofMisfoldedIsoforms.

Fernandez-Funez,P.etal.(2009)PLoSGenet.5(6):e1000507.

PubMedID:19503596Applications:Immunoprecipitation

IdentificationofPhosphorylation-DependentBindingPartnersofAquaporin-2UsingProteinMassSpectrometry.

Zwang,N.A.etal.(2009)JProteomeRes.8(3):1540-1554.

PubMedID:19209902Applications:WesternBlotControl

品牌介绍

StressMarq Biosciences Inc.位于加拿大维多利亚，是一家生物科技公司，专门从事试剂与试剂盒研究。我们有强大的国际经销商网络，主要为私人和公众客户提供经我们多次试验成功的试剂，服务范围遍及全球40多个国家。我们公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。通过定位好以上四个领域，研究人员能够运用研究相关的整套试剂工具，将研究产品重点放在热休克蛋白70和90（HSP70和HSP90）两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质（包括无内毒素制剂，而非低内毒素制剂）以及经过高级验证的抗体，产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬，跻身为高端公司。研究人员能放心使用抗体产品，因为本产品将具有免疫印迹（WB）、免疫沉淀、组织染色技术的功能，如免疫组织化学（IHC）和免疫细胞化学（ICC）。本公司提供氧化应激产品，如8OHdG（DNA受损）ELISA试剂盒，同时还提供专门的抗体，适用于非定量技术，如免疫组化。在免疫组化中，组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外，StressMarq还提供新的尖端研究工具，用于开发新的生物项目，包括全球独一无二的α B晶体蛋白ELISA试剂盒（现在是公认的乳腺癌标志物）以及热休克同源蛋白70（Hsc70）ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域，前者包括当前热门的核心领域：Nav、Cav、TRP、Kv、KCNQ、HCN，后者包括当前热门的核心领域：ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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