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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/Anti-TrpC4 Antibody [S77-15]/SMC-315D-A565/100-µg
产品编号:SMC-315D-A565
市  场 价:¥6820.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$341.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/Anti-TrpC4 Antibody [S77-15]/SMC-315D-A565/100-µg
商品介绍

Overview:

Product Name TrpC4 Antibody
Description

Mouse Anti-Rat TrpC4 Monoclonal IgG1

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ICC/IF, IP, AM
Antibody Dilution WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Rat
Immunogen Synthetic peptide amino acids 930-947 of rat TrpC4
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number S77-15
Isotype IgG1
Specificity Detects ~110kDa.
Cite This Product StressMarq Biosciences Cat# SMC-315, RRID: AB_2208049
Certificate of Analysis 1 µg/ml of SMC-315 was sufficient for detection of TrpC4 in 10 µg of COS cell lysate transiently transfected with TrpC4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names CCE1 Antibody, HTRP4 Antibody, STRPC4 Antibody, TRP4 Antibody, TRPC 4 Antibody, transient receptor potential 4 Antibody
Research Areas Cell Signaling, Ion Channels, Neuroscience, Transient Receptor Potential (TRP) Channels, Transient Receptor Potential Channels
Cellular Localization Cell membrane
Accession Number NP_001076584.1
Gene ID 84494
Swiss Prot O35119
Scientific Background Transient receptor potential cation channel, subfamily C, member 4, also known as TRPC4, is a human gene encoding a protein of the same name. They are expressed in smooth muscle and endothelial cells where they regulate membrane potential and calcium influx. TrpC4 is activated by G(q)/phospholipase C-coupled receptors, but the underlying mechanism remains elusive (1). Studies suggest TrpC4 contributes to axonal regeneration after nerve injury (2).
References 1. Otsuguro K., et al. (2008) J Biol Chem. 283(15): 10026-10036.
2. Wu D., Huang W., Richardson P.M., Pristly J.V., Liu M. (2008) J. Biol. Chem. 283(1): 416-426.

Product Images

<p>Immunohistochemistry analysis using Mouse Anti-TrpC4 Monoclonal Antibody, Clone S77-15 (SMC-315). Tissue: hippocampus. Species: Human. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC4 Monoclonal Antibody (SMC-315) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.</p>

Immunohistochemistry analysis using Mouse Anti-TrpC4 Monoclonal Antibody, Clone S77-15 (SMC-315). Tissue: hippocampus. Species: Human. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC4 Monoclonal Antibody (SMC-315) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Product Citations (2)

Other Citations

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity Human Applications: Antibody Microarray

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity Mouse Applications: Antibody Microarray

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

 ATTO 565 Fluorophore Excitation and Emission SpectraOptical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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