ProductName | UbiquitinInteractDetectionKit |
Description | Captureanddetectionofubiquitinbindingproteins |
SpeciesReactivity | SpeciesIndependent |
Platform | SpinColumn |
SampleTypes | Celllysates,Tissue |
AssayType | BindingMatrix/QuantitativeWB(WesternBlot) |
Utility | Detectionkitusedtocapture,detect,identifyandcharacteriseisolatedubiquitinbindingproteinsbyWesternblottingorproteomicanalysisinsamples. |
IncubationTime | 1hour |
NumberofSamples | 20samples |
OtherResources | KitBooklet,MSDS |
StorageTemperature | 4ºC | ||||||||||||
ShippingTemperature | BlueIce | ||||||||||||
ProductType | DetectionKits | ||||||||||||
AssayOverview | TheUbiquitinInteractDetectionkitfacilitatestheselectivecaptureanddetectionofubiquitinbindingandassociatedproteinsfromcelllysatesandtissueextracts.Thekitutilisesahighcapacity,highspecificityubiquitinmatrixtogetherwithaneasy-to-usepurificationsystemforefficientisolationofubiquitinbindingproteinswithminimalnon-specificbinding.Allowspurificationofmono-andpoly-ubiquitinbindingproteins,independentofchainlinkageorlength.AnalysisbyWesternblottingorproteomicmethodsenablesidentificationandassessmentofcapturedproteins.Kitcontainssufficientubiquitininteractmatrixtoperformupto20bindingassays. | ||||||||||||
KitOverview |
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CiteThisProduct | UbiquitinInteractDetectionKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-132) |
AlternativeNames | PolyubiquitinBDetectionKit,RPS27ADetectionKit,UBA52DetectionKit,UBBDetectionKit,UBCDetectionKit,ubiquitinBDetectionKit |
ResearchAreas | Cancer,Apoptosis,Autophagy,CardiovascularSystem,CellSignaling,Epigenetics,Neurodegeneration,Neuroscience,Post-translationalModifications,Ubiquitination |
ScientificBackground | Thecovalentattachmentofubiquitintoproteins(ubiquitination)playsafundamentalroleintheregulationofcellularfunctionthroughbiologicaleventsincludingcellcycle,differentiation,immuneresponses,DNArepair,chromatinstructure,transcription,signaltransduction,endocytosis,apoptosisanddegradationbytheproteasome,autophagyandlysosomesystems.Assuchubiquitinsignallingandtheprocessesitmediatesareessentialforthenormalfunctioningofcellsanditsdysfunctionhasbeenimplicatedinwiderangeofdiseasesincludingcancer,neurodegeneration,cardiovascularandmetabolicdisorders(1,2).Thetypeofubiquitinmodification,(monoubiquitin,multiubiquitin,polyubiquitin),substrateproteinlysineresidue(s)modifiedand,inthecaseofpolyubiquitination,thechainlengthandlysinelinkagetypecontrolthefunctionandfateofubiquitinatedproteins.Inadditionallubiquitinmediatedpathwaysalsoutilisespecificubiquitinreceptorstofacilitatetheirregulation(3,4).Ubiquitinationisachievedthroughthreeenzymaticsteps.InanATP-dependentprocess,theubiquitinE1activatingenzymecatalysestheformationofareactivethioesterbondwithubiquitin,followedbyitssubsequenttransfertotheactivesitecysteineofaubiquitinE2carrierprotein.TheselectivityoftheubiquitincascadeforaparticularsubstrateproteinreliesontheinteractionbetweentheE2conjugatingenzyme(ofwhichacellcontainsrelativelyfew)andanubiquitinE3ligase,ofwhichover600havebeenidentifiedtodate.ThespecificE2-E3pairrequiredforubiquitinationofaparticularsubstrateproteininvivomayalsocontrolthetype,pointandlength/linkage(polyubiquitin)oftheubiquitinmodification(5). |
References | 1.Fulda,S.,Rajalingam,K.&Dikic,I.EMBOmolecularmedicine4,545–56(2012). 2.Shaid,S.,Brandts,C.H.,Serve,H.&Dikic,I.Celldeathanddifferentiation20,21–30(2013). 3.Husnjak,K.&Dikic,I.Annualreviewofbiochemistry81,291–322(2012). 4.Komander,D.&Rape,M.AnnualReviewofBiochemistry81,203–229(2012). 5.Spasser,L.&Brik,A.AngewandteChemie(Internationaled.inEnglish)51,6840–62(2012). |
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StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。