ProductName | BloodUreaNitrogenDetectionKit |
Description | Quantitativecolorimetricmeasurementofureanitrogen |
SpeciesReactivity | SpeciesIndependent |
Platform | Microplate |
SampleTypes | Plasma,Saliva,Serum,TissueCultureMedia,Urine |
DetectionMethod | ColorimetricAssay |
AssayType | DirectQuantitativeAssay |
Utility | Colorimetricassayusedtoquantitativelymeasureureanitrogeninavarietyofsamples. |
Sensitivity | 0.030mg/dl |
AssayRange | 0.156-10mg/dl |
Precision | InterAssayPrecision:Threehumansampleswerefurtherdilutedinwaterandruninduplicatesintwenty-eightassaysrunovermultipledaysbyfiveoperators.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-1.29mg/dL,3.1%CVSample2-2.35mg/dL,4.3%CVSample3-5.18mg/dL,3.3%CV |
IncubationTime | 30minutes |
NumberofSamples | 88samplesinduplicate |
OtherResources | KitBooklet,MSDS |
StorageTemperature | 4ºC | |||||||||||||||
ShippingTemperature | BlueIce | |||||||||||||||
ProductType | DetectionKits | |||||||||||||||
AssayOverview | TheBloodUreaNitrogen(BUN)DetectionKitisdesignedtoquantitativelymeasureureanitrogeninavarietyofsamples.AureanitrogenstandardcalibratedtoNISTreferencematerialsisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.SamplesaremixedwithColorReagentsAandBandincubatedatroomtemperaturefor30minutes.Thecoloredproductisreadat450nm.Theconcentrationofureanitrogeninthesampleiscalculated,aftermakingasuitablecorrectionforanydilution,usingsoftwareavailablewithmostplatereaders.Theresultsareexpressedintermsofmg/dLureanitrogen.Ifsamplesaretobeexpressedintermsofmg/dLurea,thedatacanbeconvertedusingthemultiplier2.14. | |||||||||||||||
KitOverview |
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CiteThisProduct | BloodUreaNitrogenDetectionKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-213) |
AlternativeNames | BloodureanitrogenDetectionKit |
ResearchAreas | CardiovascularSystem,CellSignaling |
ScientificBackground | Ureaisaby-productofproteinmetabolismbytheliver,andisthereforeremovedfromthebloodbythekidneys.Ureafreelyfiltersthroughtheglomerulous,butisreabsorbedbytherenaltubulesinaflow-dependentfashion.Thehighertheflowrate,thegreateramountofureanitrogenisclearedfromcirculationandeliminatedthroughthekidneys.Asaresult,thelevelofcirculatingureanitrogen,alongwithserumcreatinine,servesasaprimarymeasureofkidneyfunction.NormaladultBloodUreaNitrogen(BUN)levelsshouldbebetween7and21mgureanitrogenper100mLblood(mg/dL)(1).Azotemia,poorkidneyfunction,willcauseelevatedBUNlevels(≥50mg/dL)andisassociatedwithacutekidneyfailureorinjury,severeacutepancreatitis,congestiveheartfailureorgastrointestinalbleeding(2-5).Azotemiaalsocanoccurwithdehydration,asaresultofalcoholabuse,orhighproteindiets.LowerthanexpectedBUNlevelsareusuallynotclinicallypredictive,butareprimarilyassociatedwithliverdiseaseormalnutrition,includingmalabsorptionandlowproteindiets(6).Urineandsalivaareconsideredtobeacceptablenon-invasivesamplesformeasurementofureanitrogen(7).Serumcreatinineisanothermetabolicwasteproductfreelyfilteredbytheglumerulous,butdoesnotundergotubularreabsorption.ItssteadyrateofeliminationisfrequentlyusedtogenerateanindexorratiowithBUNvaluesfornormalizedevaluations.CreatinineUrinaryDetectionKit(SKT-200)andCreatinineSerumDetectionKit(SKT-217)arealsoavailablefromStressMarqBiosciences. |
References | 1.Laboratoryreferencevalues.Ureanitrogen(BUN).Rochester,Minn.:MayoFoundationforMedicalEducationandResearch;Nov.2010. 2.Waiker,SSandJVBonventre.(2008)NephronClin.Pract.109:c192-c197. 3.AlMofleh,IA.WorldJ.Gastroent.(2008)Congestiveheartfailure.14(5):675-684. 4.Iglesiase,J.etal.(2006)Nephrol.Dial.Transplant.21:3458-3465. 5.MayoClinic.“Bloodureanitrogen(BUN)tests.”http://www.mayoclinic.com/health/blood-urea-nitrogen/MY00373/DSECTION=results 6.Lum,GandSLeal-Khouri.(1989)Clin.Chem.35(4):639-640. 7.Akai,T,etal.(1983)Clin.Chem.1983.29(10):1825-1827. |
TypicalStandardCurveforBloodUreaNitrogenDetectionKitStressXpress®–SKT-213.AssayType:CoupledEnzymeAssay.DetectionMethod:ColorimetricAssay.AssayRange:0.156–10mg/dL.
LinearitywasdeterminedbytakingtwohumanserumsampleswithknownBUNconcentrationsandmixingtheminthegivenratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.
BasophilActivation-DependentAutoantibodyandInterleukin-17ProductionExacerbateSystemicLupusErythematosus.
Pan,Q.etal.(2017)Front.Immunol.8:348.
PubMedID:N/AReactivityMouseApplications:Urine
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