ProductName | CatalaseActivityKit |
Description | Quantitativecolorimetricmeasurementofcatalaseactivity |
SpeciesReactivity | SpeciesIndependent |
Platform | Microplate |
SampleTypes | Celllysates,EDTAPlasma,Erythrocytes,HeparinPlasma,Serum,Tissue |
DetectionMethod | ColorimetricAssay |
AssayType | DirectEnzymeActivityAssay |
Utility | Colorimetricassayusedtoquantitativelymeasurecatalaseactivityinavarietyofsamples. |
Sensitivity | 0.052U/ml |
AssayRange | 0.156-5U/ml |
Precision | IntraAssayPrecision:ThreehumanserumsamplesdilutedinAssayBufferwereruninreplicatesof20inanassay.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-1.71U/mL,3.5%CVSample2-0.84U/mL,4.0%CVSample3-0.48U/mL,4.8%CVInterAssayPrecision:ThreehumanserumsamplesdilutedinAssayBufferwereruninduplicatesintwenty-oneassaysrunovermultipledaysbythreeoperators.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-1.79U/mL,11.9%CVSample2-0.94U/mL,9.8%CVSample3-0.53U/mL,12.3%CV |
IncubationTime | 45Minutes |
NumberofSamples | 89samplesinduplicate |
OtherResources | KitBooklet,MSDS |
StorageTemperature | 4ºC | |||||||||||||||||||||
ShippingTemperature | BlueIce | |||||||||||||||||||||
ProductType | ActivityKits | |||||||||||||||||||||
AssayOverview | TheCatalaseActivityKitisdesignedtoquantitativelymeasurecatalaseactivityinavarietyofsamples.Abovinecatalasestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffofthestandardcurve.SamplesaredilutedintheprovidedAssayBufferandaddedtothewellsofahalfareaclearplate.Hydrogenperoxideisaddedtoeachwellandtheplateincubatedatroomtemperaturefor30minutes.ThesuppliedColorimetricDetectionReagentisadded,followedbydilutedhorserADIshperoxidaseandincubatedatroomtemperaturefor15minutes.TheHRPreactswiththesubstrateinthepresenceofhydrogenperoxidetoconvertthecolorlesssubstrateintoapink-coloredproduct.Thecoloredproductisreadat560nm.IncreasinglevelsofcatalaseinthesamplescausesadecreaseinH2O2concentrationandareductioninpinkproduct.Theactivityofthecatalaseinthesampleiscalculatedaftermakingasuitablecorrectionforanydilution,usingsoftwareavailablewithmostplatereaders.TheresultsareexpressedintermsofunitsofcatalaseactivitypermL. | |||||||||||||||||||||
KitOverview |
| |||||||||||||||||||||
CiteThisProduct | CatalaseActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-215) |
AlternativeNames | Cas1ActivityKit,CATActivityKit,Cs1ActivityKit,MGC138422ActivityKit,MGC138424ActivityKit |
ResearchAreas | Cancer,Apoptosis,OxidativeStress |
ScientificBackground | Hydrogenperoxide,H2O2isoneofthemostfrequentlyoccurringreactiveoxygenspecies.Itisformedeitherintheenvironmentorasaby-productofaerobicmetabolism,superoxideformationanddismutation,orasaproductofoxidaseactivity.Bothexcessivehydrogenperoxideanditsdecompositionproducthydroxylradical,formedinaFenton-typereaction,areharmfulformostcellcomponents.Itsrapidremovalisessentialforallaerobicallylivingprokaryoticandeukaryoticcells(1,2).Hydrogenperoxidehowevercanactasasecondmessengerinsignaltransductionpathways,inimmunecellactivation,inflammationprocesses,cellproliferation,andapoptosis(3-5).Oneofthemostefficientwaysofremovingperoxideisthroughtheenzymecatalase,whichisencodedbyasinglegene,andishighlyconservedamongspecies(6-8).Mammals,includinghumansandmice,expresscatalaseinalltissues,andahighconcentrationofcatalasecanbefoundintheliver,kidneysanderythrocytes(9,10).Theexpressionisregulatedattranscription,post-transcriptionandpost-translationlevels6,(11).Highcatalaseactivityisdetectedinperoxisomes(12).Morerecently,shortwavelengthUVradiationhasbeenshowntoproducereactiveoxygenspecies(ROS)throughtheactionofcatalase(13).ThisresponseisthoughttoactasamechanismtoprotectDNAbyconvertingdamagingUVradiationintoROSspeciesthatcanbemetabolizedanddetoxifiedbycellularantioxidantenzymes. |
References | 1.CABIscolE,TamaritJ,RosJ.(2000)Int.Microbiol.3:3–8. 2.KirkmanHN,GaetaniGF.(2007)TrendsBiochemSci.32:44–50. 3.PeusD,etal.FreeRadic.(1999)Biol.Med.27:1197–1202. 4.RahmanI,andAdcockIM.(2006)Eur.Respir.J.28:219–242. 5.VealEA,DayAM,MorganBA.(2007)Mol.Cell.26:1–14. 6.Reimer,DL,Bailley,J,Singh,SM.(1994)Genomics.21:325–336. 7.Quan,.Korneluk,RGTropak,MB,Gravel,RA.(1986)NucleicAcidsRes.14:5321–5335. 8.Nakashima,H.,etal.(1989)Gene.79:279–288. 9.DeisSEROth,A,Dounce,AL.(1970)Physiol.Rev.50:319–375. 10.Schisler,NJ,Singh,SM.(1987)Genome.29:748–760. 11.Masters,C,Pegg,M,Crane,D.(1986)Mol.CellBiochem.70:113–120. 12.Chance,B,Sies,H,Boveris,A.(1979)Physiol.Rev.59:527–605. 13.Heck,DE,Vetrano,AM,Mariano,TM&Laskin,JD.(2003)J.Biol.Chem.278:22432–22436. |
TypicalStandardCurveforCatalaseActivityKit(EnzymeActivityAssay)StressXpress®–SKT-215.AssayType:DirectEnzyme.DetectionMethod:ColorimetricAssay.AssayRange:0.156–5U/ml.
Linearitywasdeterminedbytakingtwoserumsamples,onewithahighknowncatalaseactivityof1.163U/mLandtheotherwithalowercatalaseactivityof0.485U/mLandmixingthemingivenratios.Themeasuredactivitieswerecomparedtotheexpectedvaluesbasedontheratiosused.
CatalasecatalyzesthebreakdownofHydrogenPeroxide.
MarkersofoxidativestressinumbilicalcordbloodfromG6PDdeficientAfricannewborns.
Stadem,P.S.etal.(2017)PLoSOne.12(2):e0172980.
PubMedID:28235023ReactivityHumanApplications:Umbilicalcordblood
StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。