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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® Catalase Activity Kit/SKT-215-192/2-x-96-well
产品编号:SKT-215-192
市  场 价:¥7700.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$385.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® Catalase Activity Kit/SKT-215-192/2-x-96-well
商品介绍

Overview:

ProductNameCatalaseActivityKit
Description

Quantitativecolorimetricmeasurementofcatalaseactivity

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesCelllysates,EDTAPlasma,Erythrocytes,HeparinPlasma,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeDirectEnzymeActivityAssay
UtilityColorimetricassayusedtoquantitativelymeasurecatalaseactivityinavarietyofsamples.
Sensitivity0.052U/ml
AssayRange0.156-5U/ml
PrecisionIntraAssayPrecision:ThreehumanserumsamplesdilutedinAssayBufferwereruninreplicatesof20inanassay.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-1.71U/mL,3.5%CVSample2-0.84U/mL,4.0%CVSample3-0.48U/mL,4.8%CVInterAssayPrecision:ThreehumanserumsamplesdilutedinAssayBufferwereruninduplicatesintwenty-oneassaysrunovermultipledaysbythreeoperators.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-1.79U/mL,11.9%CVSample2-0.94U/mL,9.8%CVSample3-0.53U/mL,12.3%CV
IncubationTime45Minutes
NumberofSamples89samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeActivityKits
AssayOverviewTheCatalaseActivityKitisdesignedtoquantitativelymeasurecatalaseactivityinavarietyofsamples.Abovinecatalasestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffofthestandardcurve.SamplesaredilutedintheprovidedAssayBufferandaddedtothewellsofahalfareaclearplate.Hydrogenperoxideisaddedtoeachwellandtheplateincubatedatroomtemperaturefor30minutes.ThesuppliedColorimetricDetectionReagentisadded,followedbydilutedhorserADIshperoxidaseandincubatedatroomtemperaturefor15minutes.TheHRPreactswiththesubstrateinthepresenceofhydrogenperoxidetoconvertthecolorlesssubstrateintoapink-coloredproduct.Thecoloredproductisreadat560nm.IncreasinglevelsofcatalaseinthesamplescausesadecreaseinH2O2concentrationandareductioninpinkproduct.Theactivityofthecatalaseinthesampleiscalculatedaftermakingasuitablecorrectionforanydilution,usingsoftwareavailablewithmostplatereaders.TheresultsareexpressedintermsofunitsofcatalaseactivitypermL.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-215AClear96wellHalfAreaPlates2Plates
SKC-215BCatalaseStandard90µl
SKC-215CAssayBufferConcentrate25ml
SKC-215DHydrogenPeroxideReagent5ml
SKC-215EColorimetricDetectionReagent5ml
SKC-215FHorseradishPeroxidaseConcentrate120µl
CiteThisProductCatalaseActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-215)

BIOLOGicalDescription

AlternativeNamesCas1ActivityKit,CATActivityKit,Cs1ActivityKit,MGC138422ActivityKit,MGC138424ActivityKit
ResearchAreasCancer,Apoptosis,OxidativeStress
ScientificBackgroundHydrogenperoxide,H2O2isoneofthemostfrequentlyoccurringreactiveoxygenspecies.Itisformedeitherintheenvironmentorasaby-productofaerobicmetabolism,superoxideformationanddismutation,orasaproductofoxidaseactivity.Bothexcessivehydrogenperoxideanditsdecompositionproducthydroxylradical,formedinaFenton-typereaction,areharmfulformostcellcomponents.Itsrapidremovalisessentialforallaerobicallylivingprokaryoticandeukaryoticcells(1,2).Hydrogenperoxidehowevercanactasasecondmessengerinsignaltransductionpathways,inimmunecellactivation,inflammationprocesses,cellproliferation,andapoptosis(3-5).Oneofthemostefficientwaysofremovingperoxideisthroughtheenzymecatalase,whichisencodedbyasinglegene,andishighlyconservedamongspecies(6-8).Mammals,includinghumansandmice,expresscatalaseinalltissues,andahighconcentrationofcatalasecanbefoundintheliver,kidneysanderythrocytes(9,10).Theexpressionisregulatedattranscription,post-transcriptionandpost-translationlevels6,(11).Highcatalaseactivityisdetectedinperoxisomes(12).Morerecently,shortwavelengthUVradiationhasbeenshowntoproducereactiveoxygenspecies(ROS)throughtheactionofcatalase(13).ThisresponseisthoughttoactasamechanismtoprotectDNAbyconvertingdamagingUVradiationintoROSspeciesthatcanbemetabolizedanddetoxifiedbycellularantioxidantenzymes.
References1.CABIscolE,TamaritJ,RosJ.(2000)Int.Microbiol.3:3–8.
2.KirkmanHN,GaetaniGF.(2007)TrendsBiochemSci.32:44–50.
3.PeusD,etal.FreeRadic.(1999)Biol.Med.27:1197–1202.
4.RahmanI,andAdcockIM.(2006)Eur.Respir.J.28:219–242.
5.VealEA,DayAM,MorganBA.(2007)Mol.Cell.26:1–14.
6.Reimer,DL,Bailley,J,Singh,SM.(1994)Genomics.21:325–336.
7.Quan,.Korneluk,RGTropak,MB,Gravel,RA.(1986)NucleicAcidsRes.14:5321–5335.
8.Nakashima,H.,etal.(1989)Gene.79:279–288.
9.DeisSEROth,A,Dounce,AL.(1970)Physiol.Rev.50:319–375.
10.Schisler,NJ,Singh,SM.(1987)Genome.29:748–760.
11.Masters,C,Pegg,M,Crane,D.(1986)Mol.CellBiochem.70:113–120.
12.Chance,B,Sies,H,Boveris,A.(1979)Physiol.Rev.59:527–605.
13.Heck,DE,Vetrano,AM,Mariano,TM&Laskin,JD.(2003)J.Biol.Chem.278:22432–22436.

ProductImages

<p>Typical Standard Curve for Catalase Activity Kit (Enzyme Activity Assay) StressXpress® – SKT-215. Assay Type: Direct Enzyme. Detection Method: Colorimetric Assay. Assay Range: 0.156 – 5 U/ml.</p>

TypicalStandardCurveforCatalaseActivityKit(EnzymeActivityAssay)StressXpress®–SKT-215.AssayType:DirectEnzyme.DetectionMethod:ColorimetricAssay.AssayRange:0.156–5U/ml.

<p>Linearity was determined by taking two serum samples, one with a high known catalase activity of 1.163 U/mL and the other with a lower catalase activity of 0.485 U/mL and mixing them in given ratios. The measured activities were compared to the expected values based on the ratios used.</p>

Linearitywasdeterminedbytakingtwoserumsamples,onewithahighknowncatalaseactivityof1.163U/mLandtheotherwithalowercatalaseactivityof0.485U/mLandmixingthemingivenratios.Themeasuredactivitieswerecomparedtotheexpectedvaluesbasedontheratiosused.

<p>Catalase catalyzes the breakdown of Hydrogen Peroxide.</p>

CatalasecatalyzesthebreakdownofHydrogenPeroxide.

ProductCitations(1)

OtherCitations

MarkersofoxidativestressinumbilicalcordbloodfromG6PDdeficientAfricannewborns.

Stadem,P.S.etal.(2017)PLoSOne.12(2):e0172980.

PubMedID:28235023ReactivityHumanApplications:Umbilicalcordblood

品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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