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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® Corticosterone EIA Kit/SKT-205-96/96-well
产品编号:SKT-205-96
市  场 价:¥5740.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$287.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® Corticosterone EIA Kit/SKT-205-96/96-well
商品介绍

Overview:

ProductNameCorticosteroneEIAKit
Description

Colorimetricdetectionofcorticosterone

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesDriedFecalSamples,EDTAPlasma,HeparinPlasma,Serum,TissueCultureMedia,Urine
DetectionMethodColorimetricAssay
AssayTypeSandwichEIA(EnzymeImmunoassay)
UtilityEIAkitusedtomeasurethecorticosteronepresentinsamples.
Sensitivity18.6pg/ml
AssayRange78.125-10,000pg/ml
PrecisionIntraAssayPrecision:FourhumansamplesweredilutedwithAssayBufferandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedCorticosteroneconcentrationswere:Sample1-2460.6pg/mL,6.3%CVSample2-601.5pg/mL,6.5%CVSample3-371.6pg/mL,3.1%CVSample4-259.0pg/mL,4.8%CVInterAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninduplicatesinfourteenassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedCorticosteroneconcentrationswere:Sample1-2618.3pg/mL,7.5%CVSample2-630.1pg/mL,6.4%CVSample3-267.9pg/mL,9.9%CV
NumberofSamples39samplesinduplicate
OtherResourcesKitBooklet,MSDS,SteroidSolidExtractionProtocol

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeEIAKits
AssayOverviewTheCorticosteroneEIAkitisdesignedtoquantitativelymeasureCorticosteronepresentinserum,plasma,urine,extracteddriedfecalsamples,andtissueculturemediasamples.Thiskitmeasurestotalcorticosteroneinserumandplasmaandinextractedfecalsamples.Acorticosteronestocksolutionisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.Weprovideprotocolsonpage8toprepareassaystandardsfrom5,000to78.125pg/mLorfrom10,000to78.125pg/mL.Pleasechoosethestandardrangethatfitsyoursampleconcentrationsmostappropriately.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturesheepantibodies.Acorticosterone-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofapolyclonalantibodytocorticosteronetoeachwell.Afteranhourincubationtheplateiswashedandsubstrateisadded.Thesubstratereactswiththeboundcorticosterone-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.Theconcentrationofthecorticosteroneinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-205ACoatedClear96WellPlates1Each
SKC-205BCorticosteroneStandard125µl
SKC-205CStressXpress®CorticosteroneAntibody3ml
SKC-205D StressXpress®CorticosteroneConjugate3ml
SKC-205EAssayBuffer28ml
SKC-205FDissociationReagent1ml
SKC-205GWashBufferConcentrate30ml
SKC-205HTMBSubstrate11ml
SKC-205IStopSolution5ml
SKC-205JPlateSealer1Each
CiteThisProductCorticosteroneEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-205)

BIOLOGicalDescription

AlternativeNames(11β)-​11,​21-​dihydroxypregn-​4-​ene-​3,​20-​dioneEIAKit
ResearchAreasCancer,CellSignaling,Neuroscience,OxidativeStress
ScientificBackgroundCorticosterone(C21H30O4,Kendall’sCompound‘B’)isaglucocorticoidsecretedbythecortexoftheadrenalgland.CorticosteroneisproducedinresponsetostimulationoftheadrenalcortexbyACTHandistheprecursorofaldosterone.Corticosteroneisamajorindicatorofstressandisthemajorstresssteroidproducedinnon-humanmammals.Studiesinvolvingcorticosteroneandlevelsofstressincludeimpairmentoflongtermmemoryretrieval(1),chroniccorticosteroneelevationduetodietaryrestrictions(2)andinresponsetoburninjuries(3).Inadditiontostresslevels,corticosteroneisbelievedtoplayadecisiveroleinsleep-wakepatterns(4,5).
References1.Hupé,JM,etal.Nature,1998;394:784-787.
2.KitayskyAS,KitaiskaiaEV,WingfieldJC,PiattJF.J.Comp.Physiol,2001;171:701-709.
3.ThellinO,NoelG,KhuanaS,OgleCKandHorsemanND.Shock,2001;16(5):393-397.
4.Krame,KM.andSothernRB.Chronobiol.Int.,2001;18(6):933-945.
5.Vazquez-PalaciosG,etal.Pharmacol.BiochemBehavior,2001:70(2-3):305-310.

ProductImages

<p>Typical Standard Curve for the Corticosterone EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-205. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 78.125 – 10,000 pg/ml.</p>

TypicalStandardCurvefortheCorticosteroneEIAKit(EnzymeImmunoassay)StressXpress®–SKT-205.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:78.125–10,000pg/ml.

<p>Linearity was determined by taking two serum samples treated with Dissociation Reagent and diluted 1:50 with Assay Buffer, one with a low diluted corticosterone level of 104.6 pg/mL and one with a higher diluted level of 2,890.5 pg/mL, and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.</p>

LinearitywasdeterminedbytakingtwoserumsamplestreatedwithDissociationReagentanddiluted1:50withAssayBuffer,onewithalowdilutedcorticosteronelevelof104.6pg/mLandonewithahigherdilutedlevelof2,890.5pg/mL,andmixingthemintheratiosgivenbelow.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.

<p>Chemical structure of Corticosterone</p>

ChemicalstructureofCorticosterone

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品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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