请使用支持JavaScript的浏览器!
主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® Cortisol EIA Kit/SKT-201-96/96-well
产品编号:SKT-201-96
市  场 价:¥5220.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$261.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® Cortisol EIA Kit/SKT-201-96/96-well
商品介绍

Overview:

ProductNameCortisolEIAKit
Description

Quantitativecolorimetricdetectionofcortisol

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesDriedFecalSamples,EDTAPlasma,HeparinPlasma,Saliva,Serum,TissueCultureMedia,Urine
DetectionMethodColorimetricAssay
AssayTypeSandwichEIA(EnzymeImmunoassay)
UtilityEIAkitusedtoquantitativelymeasurecortisolpresentinsamples.
Sensitivity17.3pg/ml
AssayRange100-3200pg/ml
PrecisionIntraAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedCortisolconcentrationswere:Sample1-1174.3pg/mL,6%CVSample2-475.9pg/mL,5.6%CVSample3-177.4pg/mL,14.7%CVInterAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninduplicatesintenassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedCortisolconcentrationswere:Sample1-1188.1pg/mL,7.2%CVSample2-508.7pg/mL,6.3%CVSample3-199.7pg/mL,10.9%CV
IncubationTime90Minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS,SteroidSolidExtractionProtocol,SalivaSampleHandlingInstructions

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeEIAKits
AssayOverviewTheCortisolEIAkitisdesignedtoquantitativelymeasurecortisolpresentindriedfecalextracts,saliva,urine,serum,plasmaandtissueculturemediasamples.Thiskitmeasurestotalcortisolinextractedsamplesandinserumandplasmaandfreecortisolinsalivaandurine.Acortisolstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturemouseantibodies.Acortisol-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofamonoclonalantibodytocortisoltoeachwell.Afteran1hourincubationtheplateiswashedandsubstrateisadded.Thesubstratereactswiththeboundcortisol-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.Theconcentrationofthecortisolinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-201AClearMicrotitre96wellPlate1Plate
SKC-201BCortisolStandard125µl
SKC-201CStressXpress®CortisolAntibody3ml
SKC-201DStressXpress®CortisolConjugate3ml
SKC-201EAssayBuffer28ml
SKC-201FDissociationReagent1ml
SKC-201GWashBufferConcentrate30ml
SKC-201HTMBSubstrate11ml
SKC-201IStopSolution5ml
SKC-201JPlateSealer1Each
CiteThisProductCortisolEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-201)

BIOLOGicalDescription

AlternativeNames(11β)-11,17,21-trihydroxypregn-4-ene-3,20-dioneEIAKit
ResearchAreasCancer,CellSignaling,Neuroscience,OxidativeStress
ScientificBackgroundCortisol,C21H30O5,(hydrocortisone,compoundF)istheprimaryglucocorticoidproducedandsecretedbytheadrenalcortex.Itisoftenreferredtoasthe“stresshormone”asitisinvolvedintheresponsetostressanditaffectsbloodpressure,bloodsugarlevels,andotheractionsofstressadaptation.Immunologically,cortisolfunctionsasanimportantanti-inflammatoryandplaysaroleinhypersensitivity,immunosuppression,anddiseaseresistance(1).Inthemetabolicaspect,cortisolpromotesgluconeogenesis,liverglycogendeposition,andthereductionofglucoseutilization(2).ProductionofcortisolfollowsanACTH-dependentcircADIanrhythm,withapeaklevelinthemorninganddecreasinglevelsthroughouttheday.Mostserumcortisol,allbutabout4%,isboundtoproteinsincludingcorticosteroidbindingglobulinandserumalbumin(1,3).Onlyfreecortisolisavailabletomostreceptorsanditisthroughthesereceptorsthatphysiologicalprocessesaremodulated.Abnormalcortisollevelsarebeingevaluatedforcorrelationwithavarietyofdifferentconditions,suchasprostatecancer(4),depression(5),andschizophrenia(6).ItisalreadyknownthatabnormallevelsofcortisolareinvolvedinCushing’sSyndromeandAddision’sdisease(7).
References1.E.Friess,etal.,EurJClinInvest,2000,30,Suppl3:46-50.
2.Freeman,Scott,2002.BiologicalScience.PrenticeHall;2ndPkgedition(December30,2004).
3.C.Longscope.,J.Endocrinology,1996,,SupplS125-S127.
4.J.Herbert,Lancet,1995345,1193-1194.
5.A.Michael,etal.,Biol.Psychiatry,2000,48,989-95.
6.C.R.DequetandD.J.Wallace,CurrentOpin.Ivest.Drugs,2001,8,1045-53.
7.W.M.Jeffries,Med.Hypotheses,1998,51,114-4.

ProductImages

<p>Typical Standard Curve for the Cortisol EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-201. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 100 – 3200 pg/ml.</p>

TypicalStandardCurvefortheCortisolEIAKit(EnzymeImmunoassay)StressXpress®–SKT-201.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:100–3200pg/ml.

<p>Linearity was determined by taking two human urine samples diluted 1:140, one with a low diluted cortisol level of 163.9 pg/mL and one with a higher diluted level of 2,974.9 pg/mL and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.</p>

Linearitywasdeterminedbytakingtwohumanurinesamplesdiluted1:140,onewithalowdilutedcortisollevelof163.9pg/mLandonewithahigherdilutedlevelof2,974.9pg/mLandmixingthemintheratiosgivenbelow.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.

ProductCitations(0)

Currentlytherearenocitationsforthisproduct.

品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔