ProductName | CortisolEIAKit |
Description | Quantitativecolorimetricdetectionofcortisol |
SpeciesReactivity | SpeciesIndependent |
Platform | Microplate |
SampleTypes | DriedFecalSamples,EDTAPlasma,HeparinPlasma,Saliva,Serum,TissueCultureMedia,Urine |
DetectionMethod | ColorimetricAssay |
AssayType | SandwichEIA(EnzymeImmunoassay) |
Utility | EIAkitusedtoquantitativelymeasurecortisolpresentinsamples. |
Sensitivity | 17.3pg/ml |
AssayRange | 100-3200pg/ml |
Precision | IntraAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedCortisolconcentrationswere:Sample1-1174.3pg/mL,6%CVSample2-475.9pg/mL,5.6%CVSample3-177.4pg/mL,14.7%CVInterAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninduplicatesintenassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedCortisolconcentrationswere:Sample1-1188.1pg/mL,7.2%CVSample2-508.7pg/mL,6.3%CVSample3-199.7pg/mL,10.9%CV |
IncubationTime | 90Minutes |
NumberofSamples | 40samplesinduplicate |
OtherResources | KitBooklet,MSDS,SteroidSolidExtractionProtocol,SalivaSampleHandlingInstructions |
StorageTemperature | 4ºC | |||||||||||||||||||||||||||||||||
ShippingTemperature | BlueIce | |||||||||||||||||||||||||||||||||
ProductType | EIAKits | |||||||||||||||||||||||||||||||||
AssayOverview | TheCortisolEIAkitisdesignedtoquantitativelymeasurecortisolpresentindriedfecalextracts,saliva,urine,serum,plasmaandtissueculturemediasamples.Thiskitmeasurestotalcortisolinextractedsamplesandinserumandplasmaandfreecortisolinsalivaandurine.Acortisolstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturemouseantibodies.Acortisol-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofamonoclonalantibodytocortisoltoeachwell.Afteran1hourincubationtheplateiswashedandsubstrateisadded.Thesubstratereactswiththeboundcortisol-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.Theconcentrationofthecortisolinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders. | |||||||||||||||||||||||||||||||||
KitOverview |
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CiteThisProduct | CortisolEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-201) |
AlternativeNames | (11β)-11,17,21-trihydroxypregn-4-ene-3,20-dioneEIAKit |
ResearchAreas | Cancer,CellSignaling,Neuroscience,OxidativeStress |
ScientificBackground | Cortisol,C21H30O5,(hydrocortisone,compoundF)istheprimaryglucocorticoidproducedandsecretedbytheadrenalcortex.Itisoftenreferredtoasthe“stresshormone”asitisinvolvedintheresponsetostressanditaffectsbloodpressure,bloodsugarlevels,andotheractionsofstressadaptation.Immunologically,cortisolfunctionsasanimportantanti-inflammatoryandplaysaroleinhypersensitivity,immunosuppression,anddiseaseresistance(1).Inthemetabolicaspect,cortisolpromotesgluconeogenesis,liverglycogendeposition,andthereductionofglucoseutilization(2).ProductionofcortisolfollowsanACTH-dependentcircADIanrhythm,withapeaklevelinthemorninganddecreasinglevelsthroughouttheday.Mostserumcortisol,allbutabout4%,isboundtoproteinsincludingcorticosteroidbindingglobulinandserumalbumin(1,3).Onlyfreecortisolisavailabletomostreceptorsanditisthroughthesereceptorsthatphysiologicalprocessesaremodulated.Abnormalcortisollevelsarebeingevaluatedforcorrelationwithavarietyofdifferentconditions,suchasprostatecancer(4),depression(5),andschizophrenia(6).ItisalreadyknownthatabnormallevelsofcortisolareinvolvedinCushing’sSyndromeandAddision’sdisease(7). |
References | 1.E.Friess,etal.,EurJClinInvest,2000,30,Suppl3:46-50. 2.Freeman,Scott,2002.BiologicalScience.PrenticeHall;2ndPkgedition(December30,2004). 3.C.Longscope.,J.Endocrinology,1996,,SupplS125-S127. 4.J.Herbert,Lancet,1995345,1193-1194. 5.A.Michael,etal.,Biol.Psychiatry,2000,48,989-95. 6.C.R.DequetandD.J.Wallace,CurrentOpin.Ivest.Drugs,2001,8,1045-53. 7.W.M.Jeffries,Med.Hypotheses,1998,51,114-4. |
TypicalStandardCurvefortheCortisolEIAKit(EnzymeImmunoassay)StressXpress®–SKT-201.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:100–3200pg/ml.
Linearitywasdeterminedbytakingtwohumanurinesamplesdiluted1:140,onewithalowdilutedcortisollevelof163.9pg/mLandonewithahigherdilutedlevelof2,974.9pg/mLandmixingthemintheratiosgivenbelow.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.
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StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。