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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® Cyclic AMP CLIA Kit (High-Sensitivity)/SKT-208-96/96-well
产品编号:SKT-208-96
市  场 价:¥8480.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$424.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® Cyclic AMP CLIA Kit (High-Sensitivity)/SKT-208-96/96-well
商品介绍

Overview:

ProductNameCyclicAMPCLIAKit(High-Sensitivity)
Description

ChemiluminescentdetectionofcAMP

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesCelllysates,EDTAPlasma,HeparinPlasma,Saliva,Tissue,TissueCultureMedia,Urine
DetectionMethodChemiluminescentAssay
AssayTypeDirectCLIA(ChemiluminescentImmunoassay)
UtilityCLIAkitusedtoquantitativelymeasurecAMPpresentinsamples.
Sensitivity0.119pmol/ml
AssayRangeRegular:0.185-15pmol/ml.Acetylated:0.039-5pmol/ml
PrecisionIntraAssayPrecision(Regular):TwohumanurinesamplesweredilutedwithSampleDiluentandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-11.2pmol/mL,3.9%CVSample2-3.6pmol/mL,8.3%CVIntraAssayPrecision(Acetylated):TwohumanplasmasamplesweredilutedwithSampleDiluent,acetylatedandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-1.32pmol/mL,8.4%CVSample2-0.39[pmol10.9%CVInterAssayPrecision(Regular):TwohumanurinesamplesweredilutedwithSampleDiluentandruninduplicatesinfourteenassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-6.5pmol/mL,8.1%CVSample2-2.1pmol/mL,13.0%CVInterAssayPrecision(Acetylated):TwohumanplasmasampleweredilutedwithSampleDiluent,acetylatedandruninduplicatesinfourteenassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-1.02pmol/mL,10.1%CVSample2-0.32pmol/mL,16.5%CV
IncubationTime2hours
NumberofSamples38samplesinduplicate
OtherResourcesKitBooklet,MSDS,SalivaSampleHandlingInstructions

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeCLIAKits
AssayOverviewTheCyclicAMP(cAMP)CLIA(High-Sensitivity)kitisdesignedtoquantitativelymeasurecAMPpresentinlysedcells,EDTAandheparinplasma,urine,salivaandtissueculturemediasamples.Fortissuesamples,salivaandurine,wherethelevelsofcAMPareexpectedtoberelativelyhigh,theregularformatfortheassaycanbeused.Forplasmasamplesandsomedilutecelllysatesanoptionalacetylationprotocolcanbeused.Thiskitcanmeasureaslittleas1femtomolcAMPpersample.ThekitisuniqueinthatallsamplesandstandardsaredilutedintoanacidicSampleDiluent,whichcontainsspecialadditivesandstABIlizers,forcAMPmeasurement.Thisallowsplasma,urineandsalivasamplestobereadinanidenticalmannertolysedcells.AcidifiedsamplesofcAMParestableandendogenousphosphodiesterasesareinactivatedintheSampleDiluent.AcAMPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.AwhitemicrotiterplatecoatedwithanantibodytocapturesheepIgGisprovided.PriortotheadditionofanysamplesorstandardsaneutralizingPlatePrimersolutionisaddedtoalltheusedwells.Standardsordilutedsamples,eitherwithorwithoutacetylation,arePipettedintotheprimedwells.AcAMP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionofasheepantibodytocAMPtoeachwell.Aftera2hourincubation,theplateiswashedandthechemiluminescentsubstrateisadded.ThesubstratereactswiththeboundcAMP-peroxidaseconjugatetoproducelightThegeneratedlightisdetectedinamicrotiterplatereadercapableofreADIngluminescence.TheconcentrationofthecAMPinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-208ACoatedWhite96WellPlates1Each
SKC-208BCyclicAMPStandard125µl
SKC-208CStressXpress®CyclicAMPAntibody3ml
SKC-208DStressXpress®CyclicAMPConjugateConcentrate60µl
SKC-208EConjugateDiluent3ml
SKC-208FSampleDiluent28ml
SKC-208GPlatePrimer5ml
SKC-208HAceticAnhydride2ml
SKC-208ITriethylamine4ml
SKC-208JWashBufferConcentrate30ml
SKC-208KSubstrateSolutionA6ml
SKC-208LSubstrateSolutionB6ml
SKC-208MPlateSealer1Each
CiteThisProductCyclicAMPCLIAKit(High-Sensitivity)(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-208)

BIOLOGicalDescription

AlternativeNames3"-5"-cyclicadenosinemonophosphateCLIAKit
ResearchAreasCellSignaling,Neuroscience
ScientificBackgroundAdenosine-3’,5’-cyclicmonophosphate(cyclicAMP)isasecondmessengerthatplaysanimportantroleinintracellularregulation.Specifically,itfunctionsasamediatorofactivityofseveralkeyhormonesincludingepinephrine,glucagon,andACTH(1-4).AdenylatecyclaseisactivatedbythehormonesglucagonandadrenalineandbyGprotein.Liveradenylatecyclaserespondsmorestronglytoglucagon,andmuscleadenylatecyclaserespondsmorestronglytoadrenaline.cAMPdecompositionintoAMPiscatalyzedbytheenzymephosphodiesterase.IntheHumanMetabolomeDatabasethereare166metabolicenzymeslistedthatconvertcAMP(5).OtherbiologicalactionsofcAMPincluderegulationofinnateimmunefunctioning(6),axonregeneration(7),cancer(8),andinflammation(9).
References1.Sutherland,E.W.andRall,T.W.FractionationandCharacterizationofaCyclicAdenineRibonucleotideFormedbyTissueParticles.J.Biol.Chem.,232:1077,1958.
2.Marsh,J.M.Biol.Reprod.,14:30-53,1976.
3.Korenman,S.G.andKrall,J.F.Biol.Reprod.,16:1-17,1977.
4.Kelley,D.J.,Bhattacharyya,A.,Lahvis,G.P.,Yin,J.C.P.,Malter,J.,andDavidson,R.J.Neurosci.Biobehav.Rev.,32(8):1533-1543,2008.
5.http://www.hmdb.ca/metabolites/HMDB00058
6.Serezani,C.H.,Ballinger,M.N.,Aronoff,D.M.,andPeters-Golden,M.Am.J.Resp.CellandMol.Biol.,39(2):127,2008.
7.Hannila,S.S.,andFilbin,M.T.Exp.Neurol.,209(2):321–332,2008.
8.Shankar,D.B,Cheng,J.C.,andSakamoto,K.M.Cancer,104(9):1819-24,2005.
9.GaleaE.andFeinstein,D.L.FASEBJ.,13:2125-2137,1999.

ProductImages

<p>Regular Format Typical Standard Curve for the Cyclic AMP CLIA Kit (High-Sensitivity) (Chemiluminescent Immunoassay) StressXpress® – SKT-208. Assay Type: Direct CLIA. Detection Method: Chemiluminescent Assay. Assay Range: Regular: 0.185 – 15 pmol/ml. Acetylated: 0.039 – 5 pmol/ml.</p>

RegularFormatTypicalStandardCurvefortheCyclicAMPCLIAKit(High-Sensitivity)(ChemiluminescentImmunoassay)StressXpress®–SKT-208.AssayType:DirectCLIA.DetectionMethod:ChemiluminescentAssay.AssayRange:Regular:0.185–15pmol/ml.Acetylated:0.039–5pmol/ml.

<p>Acetylated Format Typical Standard Curve for the Cyclic AMP CLIA Kit (High-Sensitivity) (Chemiluminescent Immunoassay) StressXpress® – SKT-208. Assay Type: Direct CLIA. Detection Method: Chemiluminescent Assay. Assay Range: Regular: 0.185 – 15 pmol/ml. Acetylated: 0.039 – 5 pmol/ml.</p>

AcetylatedFormatTypicalStandardCurvefortheCyclicAMPCLIAKit(High-Sensitivity)(ChemiluminescentImmunoassay)StressXpress®–SKT-208.AssayType:DirectCLIA.DetectionMethod:ChemiluminescentAssay.AssayRange:Regular:0.185–15pmol/ml.Acetylated:0.039–5pmol/ml.

<p>Linearity was determined by taking two Jurkat cell lysate samples, one with a low cAMP level of 0.2 pmol/mL and one with a higher level of 2.5 pmol/mL, and mixing them in the ratios. The measured concentrations were compared to the expected values based on the ratios used.</p>

LinearitywasdeterminedbytakingtwoJurkatcelllysatesamples,onewithalowcAMPlevelof0.2pmol/mLandonewithahigherlevelof2.5pmol/mL,andmixingthemintheratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.

<p>Chemical structure of Cyclic AMP (cAMP)</p>

ChemicalstructureofCyclicAMP(cAMP)

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品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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