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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® Cyclic GMP EIA Kit/SKT-211-96/96-well
产品编号:SKT-211-96
市  场 价:¥8080.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$404.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® Cyclic GMP EIA Kit/SKT-211-96/96-well
商品介绍

Overview:

ProductNameCyclicGMPEIAKit
Description

QuantitativecolorimetricdetectionofcGMP

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesCelllysates,EDTAPlasma,Saliva,Tissue,TissueCultureMedia,Urine
DetectionMethodColorimetricAssay
AssayTypeDirectEIA(EnzymeImmunoassay)
UtilityEIAkitusedtoquantitativelymeasurecGMPpresentinsamples.
Sensitivity0.28pmol/ml
AssayRangeRegular:0.5-32pmol/ml.Acetylated:0.3125-20pmol/ml
PrecisionIntraAssayPrecision(Regular):ThreehumanurinesamplesweredilutedwithSampleDiluentandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-8.5pmol/mL,6.3%CVSample2-4.0pmol/mL,7.8%CVSample3-1.2pmol/mL,13.6%CVIntraAssayPrecision(Acetylated):ThreehumanurinesamplesweredilutedwithSampleDiluent,acetylatedandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-8.1pmol/mL,3.8%CVSample2-3.8pmol/mL,5.2%CVSample3-1.2pmol/mL,8.2%CVInterAssayPrecision(Regular):ThreehumanurinesamplesweredilutedwithSampleDiluentandruninduplicatesintwelveassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-8.5pmol/mL,6.5%CVSample2-4.3pmol/mL,7.0%CVSample3-1.5pmol/mL,8.3%CVInterAssayPrecision(Acetylated):ThreehumanurinesampleweresdilutedwithSampleDiluent,acetylatedandruninduplicatesintwelveassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-8.7pmol/mL,12.3%CVSample2-4.3pmol/mL,14.3%CVSample3-1.5pmol/mL,14.2%CV
NumberofSamples39samplesinduplicate
OtherResourcesKitBooklet,MSDS,SalivaSampleHandlingInstructions

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeEIAKits
AssayOverviewTheCyclicGMP(cGMP)EIAkitisdesignedtoquantitativelymeasurecGMPpresentinlysedcells,EDTAplasma,urine,salivaandtissueculturemediasamples.ForsampleswherethelevelsofcGMPareexpectedtoberelativelyhigh,theregularformatfortheassaycanbeused.ForsampleswithexpectedlowlevelsofcGMP,anoptionalacetylationprotocolcanbeused.ThekitisuniqueinthatallsamplesandstandardsaredilutedintoanacidicSampleDiluent,whichcontainsspecialadditivesandstABIlizers,forcGMPmeasurement.Thisallowsplasma,urineandsalivasamplestobereadinanidenticalmannertolysedcells.AcidifiedsamplesofcGMParestableandendogenousphosphodiesterasesareinactivatedintheSampleDiluent.AcGMPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.AclearmicrotiterplatecoatedwithanantibodytocapturemouseIgGisprovidedandaneutralizingPlatePrimersolutionisaddedtoalltheusedwells.Standardsordilutedsamples,eitherwithorwithoutacetylation,arePipettedintotheprimedwells.AcGMP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionofamousemonoclonalantibodytocGMPtoeachwell.Aftera2hourincubation,theplateiswashedandsubstrateisadded.ThesubstratereactswiththeboundcGMP-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationofthecGMPinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-211ACoatedClear96WellPlates1Each
SKC-211BCyclicGMPStandard70µl
SKC-211CStressXpress®CyclicGMPAntibody3ml
SKC-211DStressXpress®CyclicGMPConjugate3ml
SKC-211ESampleDiluent28ml
SKC-211FPlatePrimer5ml
SKC-211GAceticAnhydride2ml
SKC-211HTriethylamine4ml
SKC-211IWashBufferConcentrate30ml
SKC-211JTMBSubstrate11ml
SKC-211KStopSolution5ml
SKC-211LPlateSealer1Each
CiteThisProductCyclicGMPEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-211)

BIOLOGicalDescription

AlternativeNamescGMP;3",5"-cyclicGMPEIAKit,GuanosinecyclicmonophosphateEIAKit,Cyclic3",5"-GMP;Guanosine3",5"-cyclicphosphateEIAKit
ResearchAreasCellSignaling,Neuroscience
ScientificBackgroundGuanosine3’,5’-cyclicmonophosphate(cyclicGMP;cGMP)isacriticalandmultifunctionalsecondmessengerpresentatlevelstypically10-100foldlowerthancAMPinmosttissues.IntracellularcGMPisformedbytheactionoftheenzymeguanylatecyclaseonGTPanddegradedthroughphosphodiesterasehydrolysis(1-3).Guanylatecyclases(GC)areeithersolubleormembranebound(3,4).SolubleGCsarenitricoxideresponsive,whereasmembraneboundGCsrespondtohormonessuchasacetylcholine,insulinandoxytocin.OtherchemicalslikeSEROtoninandhistaminealsocauseanincreaseincGMPlevels(5,6).CyclicGMPregulatescellularcompositionthroughcGMP-dependentkinase,cGMP-dependentionchannelsortransporters,andthroughitshydrolyticdegradationbyphosphodiesterase(1,7)
References1.Domek-Lopacinska,K.andStrosznajder,JB.(2005)JPhysiolPharmacol56Suppl2,15-34.
2.Lucas,K.A.etal.(2000)PharmacolRev52:375-414.
3.Ashman,DF.,etal.(1963),BiochemBiophysResComm,11:330-4.
4.PotterLR,Abbey-HoschS,andDickeyDM.(2006)EndocrRev27:47-72.
5.Waldman,SAandMurad,F.(1987)PharmacolRevs,39:163-197.
6.TremblayJ,GerzerR,andHametP.(1988),Adv.2ndMessanger&PhosphoproteinRes.,22:319-383.
7.MatsumotoT,KobayashiT,andKamataK.(2003)JSmoothMuscleRes39:67-86.

ProductImages

<p>Regular Format Typical Standard Curve for the Cyclic GMP EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-211. Assay Type: Direct EIA. Detection Method: Colorimetric Assay. Assay Range: Regular: 0.5 – 32 pmol/ml. Acetylated: 0.3125 – 20 pmol/ml.</p>

RegularFormatTypicalStandardCurvefortheCyclicGMPEIAKit(EnzymeImmunoassay)StressXpress®–SKT-211.AssayType:DirectEIA.DetectionMethod:ColorimetricAssay.AssayRange:Regular:0.5–32pmol/ml.Acetylated:0.3125–20pmol/ml.

<p>Acetylated Format Typical Standard Curve for the Cyclic GMP EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-211. Assay Type: Direct EIA. Detection Method: Colorimetric Assay. Assay Range: Regular: 0.5 – 32 pmol/ml. Acetylated: 0.3125 – 20 pmol/ml.</p>

AcetylatedFormatTypicalStandardCurvefortheCyclicGMPEIAKit(EnzymeImmunoassay)StressXpress®–SKT-211.AssayType:DirectEIA.DetectionMethod:ColorimetricAssay.AssayRange:Regular:0.5–32pmol/ml.Acetylated:0.3125–20pmol/ml.

<p>Linearity was determined by taking two human urine samples, one with a low cGMP level of 0.3 pmol/mL and one with a higher level of 9.7 pmol/mL, and mixing them in ratios. The measured concentrations were compared to the expected values based on the ratios used.</p>

Linearitywasdeterminedbytakingtwohumanurinesamples,onewithalowcGMPlevelof0.3pmol/mLandonewithahigherlevelof9.7pmol/mL,andmixingtheminratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.

<p>Chemical structure of cGMP (3′,5′-cyclic GMP)</p>

ChemicalstructureofcGMP(3′,5′-cyclicGMP)

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品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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