请使用支持JavaScript的浏览器!
主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® Cystatin C EIA Kit/SKT-219-96/96-well
产品编号:SKT-219-96
市  场 价:¥8480.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$424.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® Cystatin C EIA Kit/SKT-219-96/96-well
商品介绍

Overview:

ProductNameCystatinCEIAKit
Description

ColorimetricmeasurementofhumancystatinClevels

SpeciesReactivityHuman
PlatformMicroplate
SampleTypesEDTAPlasma,HeparinPlasma,Serum,TissueCultureMedia,Urine
DetectionMethodColorimetricAssay
AssayTypeSandwichEIA(EnzymeImmunoassay)
UtilityEIAkitusedtomeasurecystatinClevelsinsamples.
Sensitivity0.058ng/ml
AssayRange0.156-10ng/ml
PrecisionIntraAssayPrecision:FourhumansamplesweredilutedwithAssayBufferandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedCystatinCconcentrationswere:Sample1-7.72ng/mL,9.1%CVSample2-5.08ng/mL,10.3%CVSample3-4.42ng/mL,7.6%CVSample4-0.88ng/mL,10.3%CVInterAssayPrecision:FourhumansamplesweredilutedwithAssayBufferandruninduplicatesintenassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedCystatinCconcentrationswere:Sample1-7.79ng/mL,8.4%CVSample2-5.48ng/mL,10.2%CVSample3-4.97ng/mL,11.1%CVSample4-1.04ng/mL,12.40%CV
IncubationTime2hours
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeEIAKits
AssayOverviewTheCystatinCEIAkitisdesignedtoquantitativelymeasurehumanCystatinCpresentinBIOLOGicalsamplesandtissueculturemedia.AhumanCystatinCstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithamonoclonalantibodytocapturetheCystatinCpresent.Aftera60minuteincubation,theplateiswashedandaperoxidaseconjugatedCystatinCmonoclonalantibodyisadded.Theplateisagainincubatedfor30minutesandwashed.Substrateisthenaddedtotheplate,whichreactswiththeboundCystatinCAntibodyConjugate.Afterathirdincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationoftheCystatinCinthesampleiscalculated,aftermakingsuitablecorrectionfordilution,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-219AClear96wellPlate1Plate
SKC-219BCystatinCStandard60µl
SKC-219CStressXpress®CystatinCConjugate5ml
SKC-219DAssayBufferConcentrate28ml
SKC-219EWashBufferConcentrate30ml
SKC-219FTMBSubstrate5ml
SKC-219GStopSolution5ml
SKC-219HPlateSealer1Each
CiteThisProductCystatinCEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-219)

BiologicalDescription

AlternativeNamesCystatin3EIAKit,gammatraceEIAKit,post-gamma-globulinorneuroendocrinebasicpolypeptideEIAKit
ResearchAreasCancer,Alzheimer"sDisease,CellSignaling,Neurodegeneration,Neuroscience
ScientificBackgroundCystatinCisanon-glycosylatedproteinoflowmolecularweight(13kDa)inthecystatinsuperfamily.CystatinCisproducedataconstantrateinallnucleatedcells,secretedfromcellsandthusfoundindetectableamountsinmostbodyfluids(1,2).CystatinCbelongstothecysteineproteinaseinhibitorgroupandisassociatedwithseveralpathologicalconditions.ImbalancebetweenCystatinCandcysteineproteinasesisassociatedwithdiseasessuchasinflammation,renalfailure,cancer,Alzheimer’sdisease,multiplesclerosisandhereditaryCystatinCamyloidangiopathy(1,3,4).Increasedlevelshavebeenfoundinautoimmunediseases,withcolorectaltumorsandmetastases,patientswithinflammationandinpatientsondialysis(5,6,7).CystatinCisremovedfrombloodplasmabyglomerularfiltrationinthekidneys.Itisreabsorbedbytheproximaltubularcellsanddegraded.ThereisalinearrelationshipbetweenthereciprocalCystatinCconcentrationinplasmaandtheglomerularfiltrationrate(GFR).CystatinCissuggestedtobeabetterMarkerforGFRthantheubiquitousserumcreatininemarkerasitsserumconcentrationisnotaffectedbyotherfactorssuchasage,genderandbodymassandCystatinChashighersensitivitytodetectareducedGFRthancreatininedetermination(2,5).LowlevelsofCystatinCarefoundwiththebreakdownoftheelasticlaminaeandatherosclerosisandaBDominalaorticaneurysm(8).ThereisevidentassociationofCystatinClevelswiththeincidenceofmyocardialinfarction,coronarydeathandanginapectoris,presentingariskfactorforsecondarycardiovascularevents(9).
References1.PergandeM.andJungK.ClinChem.199339(9):1885-90.
2.NewmanD,etal.KidneyInt’l.1995Jan;47(1):312-8.
3.LevyE.ExpertRev.Neurotherapeutics2008May;8(5):687-9.
4.NakashimaI,etal.AnnNeurol.2007Aug;62(2):197-200.
5.UchidaK.andGotoh,A.ClinChimActa.2002Sep;323:121-8.
6.PucciL,etal.ClinChem.2007Mar;53(3):480-8.
7.StabucB,etal.ClinChem.2000Feb;46(2):193-7.
8.ShlipakM,etal.NEJM2005May;352(20):2049-60.
9.KoenigW,etal.ClinChem.2005Feb;51(2):321-7.

ProductImages

<p>Typical Standard Curve for the Cystatin C EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-219. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 0.156 – 10 ng/ml.</p>

TypicalStandardCurvefortheCystatinCEIAKit(EnzymeImmunoassay)StressXpress®–SKT-219.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:0.156–10ng/ml.

<p>Serum linearity was determined by taking two human serum samples diluted 1:100, one with a low diluted Cystatin C level of 4.94 ng/mL and one with a higher diluted level of 12.31 ng/mL and mixing them in the ratios given below. Urine linearity samples were diluted 1:16. One sample with a value of 1.64 ng/mL was mixed in ratios with a sample of 3.72 ng/mL. The diluted serum and urine samples were mixed 4:1, 3:2, 2:3 and 1:4 and run in the assay. The measured concentrations were compared to the values previously determined.</p>

Serumlinearitywasdeterminedbytakingtwohumanserumsamplesdiluted1:100,onewithalowdilutedCystatinClevelof4.94ng/mLandonewithahigherdilutedlevelof12.31ng/mLandmixingthemintheratiosgivenbelow.Urinelinearitysampleswerediluted1:16.Onesamplewithavalueof1.64ng/mLwasmixedinratioswithasampleof3.72ng/mL.Thedilutedserumandurinesamplesweremixed4:1,3:2,2:3and1:4andrunintheassay.Themeasuredconcentrationswerecomparedtothevaluespreviouslydetermined.

ProductCitations(0)

Currentlytherearenocitationsforthisproduct.

品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔