StressMarq/FCR1/SIH-180-1MG/1-mg,StressMarq,,StressMarq,StressMarq Biosciences,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：StressMarq/FCR1/SIH-180-1MG/1-mg

产品编号：SIH-180-1MG

市场价：¥8500.00

场地：美国(厂家直采)

产品分类：生化试剂>缓冲液>酸碱缓冲液>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$425.00

品牌： StressMarq

公司：StressMarq

公司分类：

商品介绍

Overview:

ProductName	FCR1
Description	FRETratiometricfluorescence-basedredoxsensor
MolecularFormula	C₃₄H₃₇N₇O₆
MolecularWeight	639

Properties

StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
ProductType	RedoxProbe
Solubility	SolubleinDMSO
Source	Synthetic
Appearance	OrangeSolid
SafetyPhrases	Classification:Caution:Substancenotyetfullytested.SafetyPhrases:S22-DonotbreathedustS24/25-AvoidcontactwithskinandeyesS36/37/39-Wearsuitableprotectiveclothing,glovesandeye/faceprotection
CiteThisProduct	FCR1(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SIH-180)

BIOLOGicalDescription

AlternativeNames	7-(Diethylamino)-N-((1r,4r)-4-(2-(10-ethyl-2,4-dioxo-4,10-dihydrobenzo[g]pteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide(FCR1)
ResearchAreas	Cancer,OxidativeStress
ScientificBackground	FCR1orflavincoumarinredoxsensor1,isanovelratiometricfluorescentredoxsensorutilizedinfluorescencelifetimeimagingmicroscopyandflowcytometry.Intheoxidizedform,excitationofFCR1at405nmresultsinagreenfluorescencewithmaxemissionsat525nm.Treatmentwithamildreducingagent(includingsodiumcyanoborohydride,DTT,andglutathione)reducestheflavintherebydecreasingthegreenfluorescenceintensity,andincreasingtheblue.Re-oxidationcanbeachievedusingmildoxidizingagents.Ingeneral,theFCR1probecanbeusedtoobservechangesinoxidativecapacitywithoutinterferencefrombackgroundeffects.
References	1.KaurA.,HaghighatbinM.A.,HoganC.F.,andNewE.J.(2015)Chem.Commun.Epub.

ProductImages

<p>Chemical structure and design of FCR1 (SIH-180), showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

ChemicalstructureanddesignofFCR1(SIH-180),showingFRETprocessesinoxidisedform.Inset:photographsofcuvettesofFCR1inoxidisedandreducedformsunder365nmexcitation.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Fluorescence behavior of FCR1 (SIH-180) in the oxidized (green) and reduced (blue) forms, using 10 µM. Excitation: 405 nm. Reduced Emission: 475 nm. Oxidized Emission: 520 nm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

FluorescencebehaviorofFCR1(SIH-180)intheoxidized(green)andreduced(blue)forms,using10µM.Excitation:405nm.ReducedEmission:475nm.OxidizedEmission:520nm.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Two photon – confocal microscopy imaging of HeLa cells treated with FCR1 (SIH-180, 10 µM, 15 min, λex = 820 nm) and (a) N-acetyl cysteine (50 µM, 30 min), (b) vehicle control and (c) H2O2 (50 µM, 30 min) in blue and green channels. The pseudo colour ratio images indicate the ratio of emission intensity in the green channel to blue channel. Scale bar represents 20 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Twophoton–confocalmicroscopyimagingofHeLacellstreatedwithFCR1(SIH-180,10µM,15min,λex=820nm)and(a)N-acetylcysteine(50µM,30min),(b)vehiclecontroland(c)H2O2(50µM,30min)inblueandgreenchannels.Thepseudocolourratioimagesindicatetheratioofemissionintensityinthegreenchanneltobluechannel.Scalebarrepresents20µm.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Fluorescence lifetimes of the donor (420 – 470 nm) in HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 820 nm) and N-acetyl cysteine, vehicle control and H2O2. Pseudo-colour images represent mean lifetime. Scale bar represents 50 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Fluorescencelifetimesofthedonor(420–470nm)inHeLacellstreatedwithFCR1(SIH-180,10µM,λex=820nm)andN-acetylcysteine,vehiclecontrolandH2O2.Pseudo-colourimagesrepresentmeanlifetime.Scalebarrepresents50µm.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Flow cytometric studies of HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 405 nm) showing the fluorescence ratio (560 / 450 nm) when treated with N-acetyl cysteine (blue), vehicle control (green) and H2O2 (red). Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

FlowcytometricstudiesofHeLacellstreatedwithFCR1(SIH-180,10µM,λex=405nm)showingthefluorescenceratio(560/450nm)whentreatedwithN-acetylcysteine(blue),vehiclecontrol(green)andH2O2(red).ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

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品牌介绍

StressMarq Biosciences Inc.位于加拿大维多利亚，是一家生物科技公司，专门从事试剂与试剂盒研究。我们有强大的国际经销商网络，主要为私人和公众客户提供经我们多次试验成功的试剂，服务范围遍及全球40多个国家。我们公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。通过定位好以上四个领域，研究人员能够运用研究相关的整套试剂工具，将研究产品重点放在热休克蛋白70和90（HSP70和HSP90）两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质（包括无内毒素制剂，而非低内毒素制剂）以及经过高级验证的抗体，产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬，跻身为高端公司。研究人员能放心使用抗体产品，因为本产品将具有免疫印迹（WB）、免疫沉淀、组织染色技术的功能，如免疫组织化学（IHC）和免疫细胞化学（ICC）。本公司提供氧化应激产品，如8OHdG（DNA受损）ELISA试剂盒，同时还提供专门的抗体，适用于非定量技术，如免疫组化。在免疫组化中，组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外，StressMarq还提供新的尖端研究工具，用于开发新的生物项目，包括全球独一无二的α B晶体蛋白ELISA试剂盒（现在是公认的乳腺癌标志物）以及热休克同源蛋白70（Hsc70）ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域，前者包括当前热门的核心领域：Nav、Cav、TRP、Kv、KCNQ、HCN，后者包括当前热门的核心领域：ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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