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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® Glutathione S-Transferase Activity Kit/SKT-203/96-well
产品编号:SKT-203
市  场 价:¥7180.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$359.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® Glutathione S-Transferase Activity Kit/SKT-203/96-well
商品介绍

Overview:

ProductNameGlutathioneS-TransferaseActivityKit
Description

QuantitativefluorometricmeasurementoftheactivityofGST

SpeciesReactivityHuman,Fish,Mollusk,Oyster,Toadfish(Batrachoididae)
PlatformMicroplate
SampleTypesCelllysates,EDTAPlasma,HeparinPlasma,Serum,Urine
DetectionMethodFluorometricAssay
AssayTypeDirectEnzymeActivityAssay
UtilityFluorometricassayusedtoquantitativelymeasuretheactivityofglutathioneS-transferaseinsamples.
Sensitivity2.70mU/ml
AssayRange7.81-500mU/ml
PrecisionIntraAssayPrecision:Fourserumsampleswerediluted1:2withAssayBufferandruninreplicatesof16inanassay.ThemeanandprecisionofthecalculatedGSTactivitieswere:Sample1-315.9mU/mL,4.6%CVSample2-221.2mU/mL,5.6%CVSample3-88.2mU/mL,4.2%CVSample4-22.7mU/mL,6.6%CVInterAssayPrecision:Fourserumsampleswerediluted1:2withAssayBufferandruninduplicatesintwentyassaysovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedGSTactivitieswere:Sample1-291.7mU/mL,12.6%CVSample2-218.5mU/mL,11.0%CVSample3-89.6mU/mL,10.4%CVSample4-23.0mU/mL,15.9%CV
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeActivityKits
AssayOverviewTheGlutathioneS-TransferaseActivitykitisdesignedtoquantitativelymeasuretheactivityofGSTpresentinavarietyofsamples.AGSTstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.Thekitutilizesanon-fluorescentmoleculethatisasubstratefortheGSTenzymewhichcovalentlyattachestoglutathione(GSH)toyieldahighlyfluorescentproduct.MixingthesampleorstandardwiththesuppliedDetectionReagentandGSHandincubatingatroomtemperaturefor30minutesyieldsafluorescentproductwhichisreadat460nminafluorescentplatereaderwithexcitationat390nm.TheactivityoftheGSTinthesampleiscalculated,aftermakingasuitablecorrectionforanydilutionofthesample,usingsoftwareavailablewithmostfluorescenceplatereaders.Wehaveprovideda96wellplateformeasurementbutthisassayisadaptableforhigherdensityplateformats.TheendusershouldensurethattheirHTSblackplateissuitableforusewiththesereagentspriortorunningsamples.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-203ABlackHalfArea96WellPlate1Plate
SKC-203BGlutathioneS-TransferaseStandard50µl
SKC-203CGSTDetectionReagent1vial
SKC-203DDryDMSO2ml
SKC-203EAssayBuffer45ml
SKC-203FGlutathione(GSH)300µl
CiteThisProductGlutathioneS-TransferaseActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-203)

BIOLOGicalDescription

ProductImages

<p>Continuous Kinetic Assay Typical Standard Curve for Glutathione S-Transferase Activity Kit (Enzyme Activity Assay) StressXpress® – SKT-203. Assay Type: Direct. Detection Method: Fluorometric Assay. Assay Range: 7.81 – 500 mU/ml.</p>

ContinuousKineticAssayTypicalStandardCurveforGlutathioneS-TransferaseActivityKit(EnzymeActivityAssay)StressXpress®–SKT-203.AssayType:Direct.DetectionMethod:FluorometricAssay.AssayRange:7.81–500mU/ml.

<p>End-Point Assay Typical Standard Curve for Glutathione S-Transferase Activity Kit (Enzyme Activity Assay) StressXpress® – SKT-203. Assay Type: Direct. Detection Method: Fluorometric Assay. Assay Range: 7.81 – 500 mU/ml.</p>

End-PointAssayTypicalStandardCurveforGlutathioneS-TransferaseActivityKit(EnzymeActivityAssay)StressXpress®–SKT-203.AssayType:Direct.DetectionMethod:FluorometricAssay.AssayRange:7.81–500mU/ml.

<p>Linearity was determined by taking Jurkat cell lysates at 10 x 106 and 0.8 x 106 cells/mL and mixing in the ratios given below. The measured activities were compared to the expected values based on the ratios used.</p>

LinearitywasdeterminedbytakingJurkatcelllysatesat10x106and0.8x106cells/mLandmixingintheratiosgivenbelow.Themeasuredactivitieswerecomparedtotheexpectedvaluesbasedontheratiosused.

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品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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AlternativeNamesγ-L-Glutamyl-L-cysteinylglycineActivityKit(2S)-2-Amino-5-[[(2R)-1-(carboxymethylamino)-1-oxo-3-sulfanylpropan-2-yl]amino]-5-oxopentanoicacidActivityKit
ResearchAreasCancer,OxidativeStress
ScientificBackgroundTheGlutathioneS-Transferase(GST)familyofisozymesfunctiontodetoxifyandneutralizeawidevarietyofelectrophilicmoleculesbymediatingtheirconjugationwithreducedglutathione(1).HumanGSTsareencodedby5genefamilies,expressinginalmostalltissuesasfourcytosolicandonemicrosomalforms.Dividingthefamilybyisoelectricpoints,thebasicalpha(pI8–11),theneutralmu(pI5–7)andacidicpi(pH<5) classes are populated by additional subclasses, each isozyme displaying differential specificity for given electrophilic molecules (2). Given its pivotal role in ameliorating oxidative stress/damage, GST activity has been repeatedly investigated as a bioMarkerforarthritis,asthma,COPD,andmultipleformsofcancer,aswellasanenvironmentalmarker(3-7).ExaminationofGSTisoformsandactivityinhumancancers,tumorsandtumorcelllineshasrevealedthepredominanceoftheacidicpiclass.FurThermore,thisactivityisthoughttosubstantiallycontributetotheinnateoracquiredresistanceofspecificneoplasmstoanticancertherapy(8,9).
References1.HABIg,W,etal.J.Biol.Chem.1974249(22):7130-7139.
2.Cook,JA,etal.CancerRes.199151:1606-1612.
3.Dalle-Donne,I,etal.200652(4):601-623.
4.Surapneni,KM&VSCGopan.Ind.J.Clin.Biochem.200823(1):41.44.
5.Mohan,SK&OVenkataramana.Ind.J.Med.Sci.200761:9-14.
6.Ferrandina,G.,etal.Ann.Oncol.19978:343-350.
7.Otitoju,O&Onwarah,INE.AfricanJ.Biotech.20076(12)1455-1459.
8.Shea,TC,etal.CancerRes.198848:527-533.
9.Shea,TC,etal.CancerRes.199050:6848-6853.