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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® HSC70 ELISA kit/SKT-106-96/96-well
产品编号:SKT-106-96
市  场 价:¥12880.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$644.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® HSC70 ELISA kit/SKT-106-96/96-well
商品介绍

Overview:

ProductNameHSC70ELISAkit
Description

ColorimetricdetectionofHSC70

SpeciesReactivityHuman
PlatformMicroplate
SampleTypesCelllysates,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateHSC70concentrationinsamples.
Sensitivity1.54ng/ml
AssayRange2.34-150ng/mL
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-106AAnti-Hsc70ImmunoassayPlate1Plate
SKC-106B5XHsc70ExtractionReagent1vial/10ml
SKC-106CRecombinantHsc70Standard2vials
SKC-106DStandardandSampleDiluent1vial/50ml
SKC-106E10XWashBufferConcentrate1vial/100ml
SKC-106FAnti-Hsc70BiotinylatedAntibodyConcentrate1vial/150µl
SKC-106GAnti-Hsc70BiotinylatedAntibodyDiluent1vial/13ml
SKC-106HStreptavidin:HRPConcentrate1vial/150µl
SKC-106IStreptavidin:HRPDiluent1vial/13ml
SKC-106JTMBSubstrate1vial/13ml
SKC-106KStopSolution1vial/13ml
CiteThisProductHSC70ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-106)

BIOLOGicalDescription

AlternativeNamesHSC54ELISAKit,HSC71ELISAKit,HSC73ELISAKit,HSP71ELISAKit,HSP73ELISAKit,HSPA10ELISAKit,HSPA8ELISAKit,LAP1ELISAKit,NIP71ELISAKit
ResearchAreasCancer,HeatShock
ScientificBackgroundHSP70genesencodeabundantheat-inducible70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast5O%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingabilitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).Whencellsaresubjectedtometabolicstress(e.g.,heatshock)amemberoftheHSP70family,HSP70(HSP72),isexpressed;HSP70ishighlyrelatedtoHSC70(>90%sequenceidentity).ConstitutivelyexpressedHSC70rapidlyformsastablecomplexwiththehighlyinducIBLeHSP70incellsfollowingheatshock.TheinteractionofHSC70withHSP70isregulatedbyATP.Thesetwoheatshockproteinsmovetogetherinthecellexperiencingstress.FurThermore,researchonHSC70hasimplicatesitwitharoleinfacilitatingtherecoveryofcentrosomalstructureandfunctionafterheatshock(6).
References1.BrownC.L.,etal.(1993)J.CellBiol.,120(5):1101-1112.
2.BoorsteinW.R.,ZiegelhofferT.,andCraigE.A.(1993)J.Mol.Evol.38(1):1-17.
3.RothmanJ.(1989)Cell59:591-601.
4.DeLuca-Flaherty,etal.(1990)Cell62:875-887.
5.BorkP.,SanderC.,andValenciaA.(1992)Proc.NatlAcad.Sci.USA89:7290-7294.
6.BrownC.L.,etal.(1996)J.Biol.Chem.271(2):833-840.

ProductImages

<p>Typical Standard Curve for the HSC70 ELISA kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-106. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 2.34 – 150 ng/mL.</p>

TypicalStandardCurvefortheHSC70ELISAkit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-106.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:2.34–150ng/mL.

ProductCitations(1)

OtherCitations

ChangesintheHeatShock70kDaProteinLevelinHumanNeutrophilsInducedbyHeatShock.

Boyko,A.A.,Vetchinin,S.S.,Sapozhnikov,A.M.andKovalenko,E.I.(2014)BioorgKhim.40(5):488-498.

PubMedID:25895348ReactivityHumanApplications:Neutrophil

品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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