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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® HSP27 ELISA Kit/SKT-109-96/96-well
产品编号:SKT-109-96
市  场 价:¥10280.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$514.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® HSP27 ELISA Kit/SKT-109-96/96-well
商品介绍

Overview:

ProductNameHSP27ELISAKit
Description

ColorimetricdetectionofHSP27

SpeciesReactivityHuman
PlatformMicroplate
SampleTypesCelllysates,Plasma,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateHSP27concentrationinsamples.
Sensitivity0.04ng/ml
AssayRange0.2-13ng/ml
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-109AAnti-Hsp27ImmunoassayPlate1Plate
SKC-109B5XHsp27ExtractionReagent1vial/10ml
SKC-109CRecombinantHsp27Standard2vials
SKC-109DStandardandSampleDiluent1vial/50ml
SKC-109E10XWashBufferConcentrate1vial/100ml
SKC-109FAnti-Hsp27BiotinylatedAntibodyConcentrate1vial/150µl
SKC-109GAnti-Hsp27BiotinylatedAntibodyDiluent1vial/13ml
SKC-109HStreptavidin:HRPConcentrate1vial/150µl
SKC-109IStreptavidin:HRPDiluent1vial/13ml
SKC-109JTMBSubstrate1vial/13ml
SKC-109KStopSolution1vial/13ml
CiteThisProductHSP27ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-109)

BIOLOGicalDescription

AlternativeNames28kDaheatshockproteinELISAKit,CMT2FELISAKit,HSP25ELISAKit,HSP27ELISAKit,HSP28ELISAKit,HSPB1ELISAKit,SRP27ELISAKit
ResearchAreasCancer,HeatShock
ScientificBackgroundHSP27sbelongtoanabundantandubiquitousfamilyofsmallheatshockproteins(sHSP).ItisanimportantHSPfoundinbothnormalhumancellsandcancercells.ThebasicstructureofmostsHSPsisahomologousandhighlyconservedaminoacidsequence,withanalpha-crystallin-domainattheC-terminusandtheWD/EPFdomainatthelessconservedN-terminus.ThisN-terminusisessentialforthedevelopmentofhighmolecularoligomers(1,2).HSP27-oligomersconsistofstabledimersformedbyasmanyas8-40HSP27proteinmonomers(3).Theoligomerizationstatusisconnectedwiththechaperoneactivity:aggregatesoflargeoligomershavehighchaperoneactivity,whereasdimershavenochaperoneactivity(4).HSP27islocalizedtothecytoplasmofunstressedcellsbutcanredistributetothenucleusinresponsetostress,whereitmayfunctiontostABIlizeDNAand/orthenuclearmembrane.Otherfunctionsincludechaperoneactivity(asmentionedabove),Thermotoleranceinvivo,inhibitionofapoptosis,andsignaltransduction.Specifically,invitro,itactsasanATP-independentchaperonebyinhibitingproteinaggregationandbystabilizingpartiallydenaturedproteins,whichensuresrefoldingoftheHSP70complex.HSP27isalsoinvolvedintheapoptoticsignalingpathwaybecauseitinterfereswiththeactivationofcytochromec/Apaf-1/dATPcomplex,therebyinhibitingtheactivationofprocaspase-9.ItisalsohypothesizedthatHSP27mayservesomeroleincross-bridgeformationbetweenactinandmyosin(5).Andfinally,HSP27isalsothoughttobeinvolvedintheprocessofcelldifferentiation.Theup-regulationofHSP27correlateswiththerateofphosphorylationandwithanincreaseoflargeoligomers.ItispossIBLethatHSP27mayplayacrucialroleinterminationofgrowth(6).LookingformoreinformationonHSP27?VisitournewHSP27ScientificResourceGuideathttp://www.HSP27.com.
References1.KimK.K.,KimR.,andKim,S.(1998)Nature394(6693):595-599.
2.VanMontfortR.,SlingsbyC.,andVierlingE.(2001)AddvProteinChem.59:105-56.
3.EhrnspergerM.,GraberS.,GaestelM.andBuchnerJ.(1997)EMBOJ.16:221-229.
4.CioccaD.R.,OesterreichS.,ChamnessG.C.,McGuireW.L.,andFuguaS.A.(1993)JNatlCancerInst.85(19):1558-70.
5.SartoC.,BinnzP.A.,andMocarelliP.(2000)Electrophoresis.21(6):1218-26.
6.ArrigoA.P.(2005)JCellBiochem.94(2):241-6.

ProductImages

<p>Typical Standard Curve for the HSP27 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-109. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.2 – 13 ng/mL.</p>

TypicalStandardCurvefortheHSP27ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-109.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.2–13ng/mL.

ProductCitations(1)

OtherCitations

Elevationofserumheat-shockproteinlevelsinamyotrophiclateralsclerosis.

Miyazaki,D.etal.(2016)NeurolSci.37(8):1277-81.

PubMedID:27112486ReactivityHumanApplications:Serum

品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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