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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® HSP60 ELISA Kit/SKT-110-96/96-well
产品编号:SKT-110-96
市  场 价:¥10280.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$514.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® HSP60 ELISA Kit/SKT-110-96/96-well
商品介绍

Overview:

ProductNameHSP60ELISAKit
Description

ColorimetricdetectionofHSP60

SpeciesReactivityHuman
PlatformMicroplate
SampleTypesCelllysates,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateHSP60concentrationinsamples.
Sensitivity1.73ng/ml
AssayRange11-700ng/ml
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBookletLotNo.BH585599,KitBookletLotNo.TH585578,KitBookletLotNo.160427,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateat37°Cfor1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateat37°Cfor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-110AAnti-Hsp60ImmunoassayPlate1Plate
SKC-110B5XHsp60ExtractionReagent1vial/10ml
SKC-110CRecombinantHsp60Standard1vial
SKC-110DStandardandSampleDiluent1vial/50ml
SKC-110E10XWashBufferConcentrate1vial/100ml
SKC-110FAnti-Hsp60BiotinylatedAntibodyConcentrate1vial/150µl
SKC-110GAnti-Hsp60BiotinylatedAntibodyDiluent1vial/13ml
SKC-110HStreptavidin:HRPConcentrate1vial/150µl
SKC-110IStreptavidin:HRPDiluent1vial/13ml
SKC-110JTMBSubstrate1vial/13ml
SKC-110KStopSolution1vial/13ml
CiteThisProductHSP60ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-110)

BIOLOGicalDescription

AlternativeNamesCPN60ELISAKit,GROELELISAKit,HLD4ELISAKit,HSP60ELISAKit,HSP65ELISAKit,HSPD1ELISAKit,HuCHA60ELISAKit,SPG13ELISAKit
ResearchAreasCancer,HeatShock
ScientificBackgroundInbothprokaryoticandeukaryoticcells,themisfoldingandaggregationofproteinsduringbiogenesisandunderconditionsofcellularstressarepreventedbymolecularchaperones.MembersoftheHSP60familyofheatshockproteinsaresomeofthebestcharacterizedchaperones.HSP60,alsoknownasCpn60orGroEl,isanabundantproteinsynthesizedconstitutivelyinthecellthatisinducedtoahigherconcentrationafterbriefcellshock.Itispresentinmanyspeciesandexhibitsaremarkablesequencehomologyamongvariouscounterpartsinbacteria,plants,andmammalswithmorethanhalfoftheresiduesidenticalbetweenbacterialandmammalianHSP60(1-3).WhereasmammalianHSP60islocalizedwithinthemitochondria,plantHSP60,orotherwiseknownasRubisco-bindingprotein,islocatedinplantchloroplasts.IthasbeenindicatedthattheseproteinscarryoutaveryimportantbiologicalfunctionduetothefactthatHSP60ispresentinsomanydifferentspecies.ThecommoncharacteristicsoftheHSP60sfromthedivergentspeciesarei)highabundance,ii)inductionwithenvironmentalstresssuchasheatshock,iii)homo-oligomericstructuresofeither7or14subunitswhichreversIBLydissociateinthepresenceofMg2+andATP,iv)ATPaseactivityandv)aroleinfoldingandassemblyofoligomericproteinstructures(4).Thesesimilaritiesaresupportedbyrecentstudieswherethesingle-ringhumanmitochondrialhomolog,HSP60withitsco-chaperonin,HSP10wereexpressedinaE.colistrain,engineeredsothatthegroEoperonisunderstrictregulatorycontrol.ThisstudyhasdemonstratedthatexpressionofHSP60-HSP10wasabletocarryoutallessentialinvivofunctionsofGroELanditsco-chaperonin,GroES(5).AnotherimportantfunctionofHSP60andHSP10istheirprotectivefunctionsagainstinfectionandcellularstress.HSP60hashoweverbeenlinkedtoanumberofautoimmunediseases,aswellasAlzheimers,coronaryarterydiseases,MS,anddiabetes(6-9).
References1.HartlF.U.(1996)Nature.381:571-579.
2.BukauB.andHorwich,A.L.(1998)Cell.92:351-366.
3.HartlF.U.andHayer-Hartl,M.(2002)Science.295:1852-1858.
4.JindalS.,etal.(1989)MolecularandCellularBiology.9:2279-2283.
5.LaVerda,D.,etal(1999)InfectDis.Obstet.Gynecol.7:64-71.
6.ItohH.,etal.(2002)Eur.J.Biochem.269:5931-5938.
7.GuptaS.andKnowltonA.A.J.CellMol.Med.9:51-58.
8.DeocarisC.C.,etal.(2006)CellStressChaperones.11:116-128.
9.LaiH.C.,etal.(2007)Am.J.Physiol.Endocrinol.Metab.292:E292-E297.

ProductImages

<p>Typical Standard Curve for the HSP60 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-110. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 7 – 450 ng/mL.</p>

TypicalStandardCurvefortheHSP60ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-110.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:7–450ng/mL.

<p>Typical Standard Curve for the HSP60 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-110. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 11 – 700 ng/ml.</p>

TypicalStandardCurvefortheHSP60ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-110.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:11–700ng/ml.

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品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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