Overview:
| ProductName | HSP70ELISAKit |
| Description | ColorimetricdetectionofHSP70 |
| SpeciesReactivity | Dog,Human,Goat |
| Platform | Microplate |
| SampleTypes | Celllysates,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateHSP70concentrationinsamples. |
| Sensitivity | 0.18ng/ml |
| AssayRange | 0.781-50ng/mL |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | |||||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | |||||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | |||||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardtoappropriatewells.3.Add50µLofPre-TreatmentBuffertoallsamplewells.4.Add50µLofsampletoappropriatewells.5.CoverplatewithPlateSealerandincubateat37°Cfor2hours.6.Washplatefourtimeswith1XWashBuffer.7.Add100µLofDetectionAntibodyWorkingSolutiontoeachwell.8.CoverplatewithPlateSealerandincubateat37°Cfor2hours.9.Washplatefourtimeswith1XWashBufferasdescribedinstep6.10.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.11.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.12.Washplatefourtimeswith1XWashBufferasdescribedinstep6.13.Add100µLofTMBSubstratetoeachwell.14.Developtheplateinthedarkatroomtemperaturefor30minutes.15.Stopreactionbyadding100µLofStopSolutiontoeachwell.16.Measureabsorbanceonaplatereaderat450nm. | |||||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | HSP70ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-105) |
BIOLOGicalDescription
| AlternativeNames | HSP701ELISAKit,HSP702ELISAKit,HSP70.1ELISAKit,HSP72ELISAKit,HSPA1ELISAKit,HSPA1AELISAKit,HSPA1BELISAKit |
| ResearchAreas | Cancer,HeatShock |
| ScientificBackground | HSP70genesencodeabundantheat-inducIBLe70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast5O%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com. |
| References | 1.ZhoJ.(1998)Cell.94:471-480. 2.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1):1-17. 3.RothmanJ.(1989)Cell.59:591-601. 4.DeLuca-Flahertyetal.(1990)Cell.62:875-887. 5.BorkP.,SanderC.&ValenciaA.(1992)Proc.NatlAcad.Sci.USA.89:7290-7294. 6.FinkA.L.(1999)Physiol.Rev.79:425-449. 7.SmithD.F.,etal.(1993)Mol.Cell.Biol.13(2):869-876. 8.PrapapanichV.,etal.,(1996)Mol.Cell.Biol.16(11):6200-6207. 9.Fernandez-Funezetal.,(2000)Nature.408(6808):101-106. |
ProductImages
TypicalStandardCurvefortheHSP70ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-105.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.781–50ng/mL.
ProductCitations(4)
OtherCitations
Urinaryheatshockprotein72asabioMarkerofacutekidneyinjuryindogs.
Bruchim,Y.etal.(2017)VetJ.[Epubaheadofprint].
PubMedID:N/AReactivityDogApplications:Urine
Identificationofheatstress-susceptibleand-tolerantphenotypesingoatsinsemiaridtropics.
Rout,P.K.,Kaushik,R.,Ramachandran,N.andJindal,S.K.(2017)AnimalProductionScience.[Epubaheadofprint].
PubMedID:N/AReactivityGoat
Differentialexpressionpatternofheatshockprotein70geneintissuesandheatstressphenotypesingoatsduringpeakheatstressperiod.
Rout,P.K.,Kaushik,R.andRamachandran,N.(2016)CellStressChaperones.21(4):645-51.
PubMedID:27169748ReactivityGoatApplications:Varioustissues
EnterococcusfaeciumNCIMB10415ModulatesEpithelialIntegrity,HeatShockProtein,andProinflammatoryCytokineResponseinIntestinalCells.
Klingspor,S.etal.(2014)MediatorsInflamm.2015:304149.
PubMedID:25948884ReactivityHumanApplications:Epithelialintestinalcellline
StressMarq Biosciences Inc.位于加拿大维多利亚，是一家生物科技公司，专门从事试剂与试剂盒研究。我们有强大的国际经销商网络，主要为私人和公众客户提供经我们多次试验成功的试剂，服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。 通过定位好以上四个领域，研究人员能够运用研究相关的整套试剂工具，将研究产品重点放在热休克蛋白70和90（HSP70和HSP90）两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质（包括无内毒素制剂，而非低内毒素制剂）以及经过高级验证的抗体，产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬，跻身为高端公司。研究人员能放心使用抗体产品，因为本产品将具有免疫印迹（WB）、免疫沉淀、组织染色技术的功能，如免疫组织化学（IHC）和免疫细胞化学（ICC）。 本公司提供氧化应激产品，如8OHdG（DNA受损）ELISA试剂盒，同时还提供专门的抗体，适用于非定量技术，如免疫组化。在免疫组化中，组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外，StressMarq还提供新的尖端研究工具，用于开发新的生物项目，包括全球独一无二的α B晶体蛋白ELISA试剂盒（现在是公认的乳腺癌标志物）以及热休克同源蛋白70（Hsc70）ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域，前者包括当前热门的核心领域：Nav、Cav、TRP、Kv、KCNQ、HCN，后者包括当前热门的核心领域：ENaC、NCC和NKCC2以及主要水通道蛋白载体。
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