ProductName | HSP70ELISAKit(High-Sensitivity) |
Description | High-SensitivityColorimetricdetectionofHSP70 |
SpeciesReactivity | Dog,Human,Monkey,Mouse,Rat |
Platform | Microplate |
SampleTypes | Celllysates,Plasma,Serum,Tissue |
DetectionMethod | ColorimetricAssay |
AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
Utility | ELISAkitusedtoquantitateHSP70concentrationinsamples. |
Sensitivity | 0.02ng/ml |
AssayRange | 0.55-35ng/ml |
IncubationTime | 30minutes |
NumberofSamples | 40samplesinduplicate |
OtherResources | KitBookletLotNo.TH585532,MSDS |
StorageTemperature | 4ºC | |||||||||||||||||||||||||||||||||
ShippingTemperature | BlueIce | |||||||||||||||||||||||||||||||||
ProductType | ELISAKits | |||||||||||||||||||||||||||||||||
AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateat37°Cfor2hours.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatat37°Cfor2hours.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateat37°Cfor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | |||||||||||||||||||||||||||||||||
KitOverview |
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CiteThisProduct | HSP70ELISAKit(High-Sensitivity)(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-108) |
AlternativeNames | HSP701ELISAKit,HSP702ELISAKit,HSP70.1ELISAKit,HSP72ELISAKit,HSPA1ELISAKit,HSPA1AELISAKit,HSPA1BELISAKit |
ResearchAreas | Cancer,HeatShock |
ScientificBackground | HSP70genesencodeabundantheat-inducIBLe70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast5O%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com. |
References | 1.ZhoJ.(1998)Cell.94:471-480. 2.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1)1-17. 3.RothmanJ.(1989)Cell59:591-601. 4.DeLuca-Flahertyetal.(1990)Cell.62:875-887. 5.BorkP.,SanderC.&ValenciaA.(1992)Proc.NatlAcad.Sci.USA.89:7290-7294. 6.FinkA.L.(1999)Physiol.Rev.79:425-449. 7.SmithD.F.,etal.(1993)Mol.Cell.Biol.13(2):869-876. 8.PrapapanichV.,etal.(1996)Mol.Cell.Biol.16(11):6200-6207. 9.Fernandez-Funezetal.(2000)Nature.408(6808):101-106. |
TypicalStandardCurvefortheHSP70ELISAKit(High-Sensitivity)(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-108.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.55–35ng/mL.
EffectsofheatedhydrotherapyonmuscleHSP70andglucosemetabolisminoldandyoungvervetmonkeys.
Kavanagh,K.,Davis,A.T.,Jenkins,K.A.andFlynn,D.M.(2016)CellStressChaperones.21(4):717-25.
PubMedID:27188431ReactivityMonkeyApplications:Plasma
Elevationofserumheat-shockproteinlevelsinamyotrophiclateralsclerosis.
Miyazaki,D.etal.(2016)NeurolSci.37(8):1277-81.
PubMedID:27112486ReactivityHumanApplications:Serum
TheBrewedRiceVinegarKurozuIncreasesHSPA1AExpressionandAmelioratesCognitiveDysfunctioninAgedP8Mice.
Kanouchi,H.etal.(2016)PLoSONE.11(3):e0150796.
PubMedID:26943920ReactivityMouseApplications:Brainhomogenates
MuscleHeatShockProtein70PredictsInsulinResistanceWithAging.
Chichester,L.,Wylie,A.T.,Craft,S.,andKavanagh,K.(2014)JGerontolABiolSciMedSci.70(2):155-62.
PubMedID:24532784ReactivityAfricangreenmonkeyApplications:Plasmaandskeletalmuscle,andPBMC"s
StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。