ProductName | HSP90AlphaELISAkit |
Description | ColorimetricdetectionofHSP90Alpha |
SpeciesReactivity | Human,Goat |
Platform | Microplate |
SampleTypes | Celllysates,Serum,Tissue |
DetectionMethod | ColorimetricAssay |
AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
Utility | ELISAkitusedtoquantitateHSP90alphaconcentrationinsamples. |
Sensitivity | 0.117ng/ml |
AssayRange | 0.44-28ng/ml |
IncubationTime | 30minutes |
NumberofSamples | 40samplesinduplicate |
OtherResources | KitBookletLotNo.SH585170,KitBookletLotNo.1008,MSDS |
StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||
ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||
ProductType | ELISAKits | ||||||||||||||||||||||||||||||||||||
AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateat37°Cfor1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperature,20-25°Cfor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | ||||||||||||||||||||||||||||||||||||
KitOverview |
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CiteThisProduct | HSP90alphaELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-107) |
AlternativeNames | HSP86ELISAKit,HSP89AELISAKit,HSP90AELISAKit,HSP90AA1ELISAKit,HSPC1ELISAKit,HSPCAELISAKit,HsoCAL3ELISAKit,HSP90alphaELISAKit |
ResearchAreas | Cancer,HeatShock |
ScientificBackground | HSP90isahighlyconservedandessentialstressproteinthatisexpressedinalleukaryoticcells.Fromafunctionalperspective,HSP90participatesinthefolding,assembly,maturation,andstABIlizationofspecificproteinsasanintegralcomponentofachaperonecomplex(1-4).Despiteitslabelofbeingaheat-shockprotein,HSP90isoneofthemosthighlyexpressedproteinsinunstressedcells(1–2%ofcytosolicprotein).Itcarriesoutanumberofhousekeepingfunctions–includingcontrollingtheactivity,turnover,andtraffickingofavarietyofproteins.MostoftheHSP90-regulatedproteinsthathavebeendiscoveredtodateareinvolvedincellsignaling(5-6).ThenumberofproteinsnowknowntointeractwithHSP90isabout100.Targetproteinsincludethekinasesv-Src,Wee1,andc-Raf,transcriptionalregulatorssuchasp53andsteroidreceptors,andthepolymerasesofthehepatitisBvirusandtelomerase(3).WhenboundtoATP,HSP90interactswithco-chaperonesCdc37,p23,andanassortmentofimmunophilin-likeproteins,formingacomplexthatstabilizesandprotectstargetproteinsfromproteasomaldegradation.Inmostcases,HSP90-interactingproteinshavebeenshowntoco-precipitatewithHSP90whencarryingoutimmune-oadsorptionstudies,andtoexistincytosolicheterocomplexeswithit.Inanumberofcases,variationsinHSP90expressionorHSP90mutationhasbeenshowntodegradesignalingfunctionviatheproteinortoimpairaspecificfunctionoftheprotein(suchassteroidbinding,kinaseactivity)invivo.Ansamycinantibiotics,suchasgeldanamycinandrADIcicol,inhibitHSP90function(7).LookingformoreinformationonHSP90?VisitournewHSP90ScientificResourceGuideathttp://www.HSP90.ca. |
References | 1.ArlanderS.J.H.,etal.(2003)JBiolChem.278:52572-52577. 2.PearlH.,etal.(2001)AdvProteinChem.59:157-186. 3.NeckersL,etal.(2002)TrendsMolMed.8:S55-S61. 4.PrattW.,ToftD.(2003)ExpBiolMed.228:111-133. 5.PrattW.,ToftD.(1997)EndocrRev.18:306-360. 6.PrattW.B.(1998)ProcSocExptlBiolMed.217:420-434. 7.WhitesellL.,etal.(1994)ProcNatlAcadSciUSA.91:8324-8328. |
TypicalStandardCurvefortheHSP90AlphaELISAkit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-107.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.44–28ng/mL.
Identificationofheatstress-susceptIBLeand-tolerantphenotypesingoatsinsemiaridtropics.
Rout,P.K.,Kaushik,R.,Ramachandran,N.andJindal,S.K.(2017)AnimalProductionScience.
PubMedID:N/AReactivityGoat
Elevationofserumheat-shockproteinlevelsinamyotrophiclateralsclerosis.
Miyazaki,D.etal.(2016)NeurolSci.37(8):1277-81.
PubMedID:27112486ReactivityHumanApplications:Serum
Highexpressionofheatshockprotein90alphaanditssignificanceinhumanacuteleukemiacells.
Tian,W.L.etal.(2014)Gene.542(2):122-8.
PubMedID:24680776ReactivityHumanApplications:Plasma
StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。