ProductName | NitrotyrosineELISAKit |
Description | ColorimetricdetectionofNitrotyrosine |
SpeciesReactivity | SpeciesIndependent |
Platform | Microplate |
SampleTypes | Celllysates,Plasma,Serum,Urine |
DetectionMethod | ColorimetricAssay |
AssayType | CompetitiveELISA(Enzyme-linkedImmunosorbentAssay) |
Utility | ELISAkitusedtoquantitateNitrotyrosineinsamples. |
Sensitivity | 50nM |
AssayRange | 62.5-8000nM |
Precision | Intra-AssayPrecision:Threesamplesofknownconcentrationwereassayedthirtytimesononeplate.Theintra-assaycoefficientofvariationoftheNitrotyrosineELISAhasbeendeterminedtobe<10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%. |
IncubationTime | 1hour |
NumberofSamples | 38samplesinduplicate |
OtherResources | KitBooklet,MSDS,CalculationsWorksheet |
StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||
ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||
ProductType | ELISAKits | ||||||||||||||||||||||||||||||
AssayOverview | 1.PreparestandardandsamplesintheSampleandStandardDiluent.2.Add50µLofpreparedstandardsandsamplesintriplicatetoappropriatewells.3.Add50µLofthedilutedantibodypreparationtotheappropriatewells.4.CoverplatewithPlateCoverandincubateatroomtemperature(20-25°C)for1hour.5.Washplate4timeswith1XWashBuffer.6.Add100µLofTMBSubstratetoeachwell.7.Coverplateanddeveloptheplateinthedarkatroomtemperaturefor30minutes.8.Add100µLofStopSolutiontoeachwell.9.Measureabsorbanceonaplatereaderat450nm.10.Plotthestandardcurveandcalculatesampleconcentrations. | ||||||||||||||||||||||||||||||
KitOverview |
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CiteThisProduct | NitrotyrosineELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-126) |
AlternativeNames | NitrotyrosineELISAKit,3-NitrotyrosineELISAKit,3-Nitro-L-tyrosineELISAKit,3NitrotyrosineELISAKit,3-NTELISAKit,3NTELISAKit |
ResearchAreas | Cancer,Alzheimer"sDisease,CellSignaling,Neurodegeneration,Neuroscience,Nitration,OxidativeStress,Parkinson"sDisease,Post-translationalModifications |
ScientificBackground | NitrotyrosinehasbeenidentifiedasaMarkerofinflammationandNOproduction.NitrotyrosineisformedinpresenceoftheactivemetaboliteNO.Variouspathwaysincludingtheformationofperoxinitriteleadtonitrotyrosineproduction.Sincenitrotyrosineisastableendproductofperoxynitriteoxidation,assessmentofitsplasmaconcentrationmaybeusefulasamarkerofNO-dependentdamageinvivo.SinceNOXisonlyanindicatorforenhancedNOproduction,proteinassociatednitrotyrosinemightbeamoresuitablemarkerfordamageinducedbyreactivenitrogenintermediatesderivedfromNO.FurThermore,mostproteinshavealongerhalflifeinthecirculationthanNOXlevels.Thepresenceofnitrotyrosinehasbeendetectedinvariousinflammatoryprocessesincludingatheroscleroticplaques,celiacdisease,rheumatoidarthritis,chronicrenalfailureandsepticshock.Innormalplasmalow,undetectable,levelsofnitrotyrosinearepresent.Nitrosylationoftheaminoacidtyrosineoccursbothforfreetyrosineandforproteinboundtyrosine. |
TypicalStandardCurvefortheNitrotyrosineELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-126.AssayType:CompetitiveELISA.DetectionMethod:ColorimetricAssay.AssayRange:62.5–8000nM.
DiagramoftheCompetitiveELISA
DiagramofthePreparationofNitrotyrosineStandards
DiagramoftheTriplicateSamplePlateFormat
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StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。