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主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
℡ 4000-520-616
℡ 4000-520-616
StressMarq/StressXpress® Rat GRP78 ELISA Kit/SKT-137-96/96-well
产品编号:SKT-137-96
市  场 价:¥11200.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$560.00
品      牌: StressMarq
公      司:StressMarq
公司分类:
StressMarq/StressXpress® Rat GRP78 ELISA Kit/SKT-137-96/96-well
商品介绍

Overview:

ProductNameRatGRP78ELISAKit
Description

ColorimetricdetectionofGRP78inratlysates

SpeciesReactivityRat
PlatformMicroplate
SampleTypesTissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateGRP78concentrationinratsamples.
Sensitivity0.15ng/ml
AssayRange0-50ng/ml
PrecisionIntra-AssayPrecision:Threesamplesofknownconcentrationwereassayedthirtytimesononeplate.Theintra-assaycoefficientofvariationoftheNitrotyrosineELISAhasbeendeterminedtobe<10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%.
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateat37°Cfor2hours.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatat37°Cfor2hours.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidinPolyHRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateat37°Cfor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-137AAnti-RatGRP78ImmunoassayPlate1Plate
SKC-137BRecombinantRatGRP78Standard2vials/56.7ng
SKC-0001StandardandSampleDiluent1vial/50mL
SKC-0002Anti-RatGrp78BiotinylatedAntibodyConcentrate1vial/150µL
SKC-000310XWashBufferConcentrate1vial/100mL
SKC-137FAnti-RatGRP78BiotinylatedAntibodyDiluent1vial/13mL
SKC-137GStreptavidinPolyHRPConcentrate1vial/150µL
SKC-137HStreptavidinPolyHRPDiluent1vial/13mL
SKC-0004TMBSubstrate1vial/13mL
SKC-0005StopSolution1vial/13mL
SKC-0009PlateCover2
CiteThisProductRatGRP78ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-137)

BIOLOGicalDescription

AlternativeNamesGrp78ELISAKit,BindingimmunoglobulinproteinELISAKit,BiPELISAKit,78kDaglucose-regulatedproteinELISAKit,heatshock70kDaprotein5ELISAKit
ResearchAreasCancer,HeatShock,CellSignaling,Chaperones,OrganelleMarkers,Trafficking
ScientificBackgroundGRP78isaubiquitouslyexpressed,78-kDaglucose-regulatedprotein,andiscommonlyreferredtoasanimmunoglobinchainbindingprotein(BiP).TheBiPproteinsarecategorizedasstressresponseproteinsbecausetheyplayanimportantroleintheproperfoldingandassemblyofnascentproteinandinthescavengingofmisfoldedproteinsintheendoplasmicreticulumlumen.TranslationofBiPisdirectedbyaninternalribosomalentrysite(IRES)inthe5’nontranslatedregionoftheBiPmRNA.BiPIRESactivityincreaseswhencellsareheatstressed(1).GRP78isalsocriticalformaintenanceofcellhomeostasisandthepreventionofapoptosis(2).Luoetal.haveprovidedfindingsthatsuggestGRP78isessentialforembryoniccellgrowthandpluripotentcellsurvival(3).Intermsofdiseases,GRP78hasbeenshowntobeareliablebiomarkerofhypoglycemia,toserveaneuroprotectivefunctioninneuronsexposedtoglutamateandoxidativestress(4),anditsproteinlevelsarereducedinthebrainsofAlzheimer’spatients(5).Also,theinductionoftheGRP78proteinthatresultsinsevereglucoseandoxygendeprivationcouldpossIBLeleadtodrugresistancetoanti­tumordrugs(6,7).
References1.ChoS.,etal.(2007).MolCellBiol27(1):368-83.
2.YangY.,etal.(1998)JBiolChem273:25552-25555.
3.LuoS.,etal(2006)26(15):5688-97.
4.YuZ.,etal.(1999)ExpNeurol.15:302-314.
5.KoomagiR.,etal.(1999)AnticancerRes.19:4333-4336.
6.LaquerreS.,etal.(1998)J.Virology72:4940-4949.
7.DongD.,etal.(2005)CancerRes65(13):5785-91.

ProductImages

<p>Typical Standard Curve for the Rat GRP78 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-137. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0 – 50 ng/ml.</p>

TypicalStandardCurvefortheRatGRP78ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-137.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0–50ng/ml.

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品牌介绍

    StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。

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