ProductName | RetinolBindingProteinUrinaryEIAKit |
Description | ColorimetricmeasurementofRBPlevelsinurine |
SpeciesReactivity | Dog,Human,Monkey,Rat |
Platform | Microplate |
SampleTypes | Urine |
DetectionMethod | ColorimetricAssay |
AssayType | SandwichEIA(EnzymeImmunoassay) |
Utility | EIAkitusedtomeasureRBPlevelsinurinesamples. |
Sensitivity | 2.90ng/ml |
AssayRange | 3.906-1000ng/ml |
Precision | IntraAssayPrecision:Fourhumanurinesampleswerediluted1:2withAssayBufferandruninreplicatesof8inanassay.ThemeanandprecisionofthecalculatedRBPconcentrationswere:Sample1-14.8ng/mL,4.5%CVSample2-28.0ng/mL,7.5%CVSample3-53.5ng/mL,7.3%CVSample4-323.7ng/mL,2.1%CVInterAssayPrecision:Fourhumanurinesampleswerediluted1:2withAssayBufferandruninduplicatesintwenty-oneassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedRBPconcentrationswere:Sample1-14.4ng/mL,9.1%CVSample2-28.0ng/mL,9.2%CVSample3-50.8ng/mL,8.1%CVSample4-309.2ng/mL,14.0%CV |
IncubationTime | 90Minutes |
NumberofSamples | 41samplesinduplicate |
OtherResources | KitBooklet,MSDS |
StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||
ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||
ProductType | EIAKits | ||||||||||||||||||||||||||||||
AssayOverview | TheRetinolBindingProteinUrinaryEIAKitisdesignedtoquantitativelymeasureRBPpresentinurinesamples.ARBPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturerabbitantibodies.ARBP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionoftheRBPpolyclonalantibodytoeachwell.Afteranhourincubationtheplateiswashedandsubstrateisadded.ThesubstratereactswiththeboundRBP-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationoftheRBPinthesampleiscalculated,aftermakingasuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders. | ||||||||||||||||||||||||||||||
KitOverview |
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CiteThisProduct | RetinolBindingProteinUrinaryEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-218) |
AlternativeNames | CellularretinolbindingproteinEIAKit,CRBPEIAKit,CRBPEIAKit,CRBP1EIAKit,CRBP2EIAKit,RBP1EIAKit,RBP2EIAKit,RBP4EIAKit,RBPCEIAKit,Retinolbindingprotein1EIAKit,Retinolbindingprotein2EIAKit,Retinolbindingprotein4EIAKit |
ResearchAreas | CardiovascularSystem,CellSignaling |
ScientificBackground | Retinolbindingprotein(RBP)isfromafamilyofstructurallyrelatedproteinsthatbindsmallhydrophobicmoleculessuchasbilepigments,steroids,odorants,etc(1).RBPisa21kDahighlyconserved,single-chainglycoprotein,consistingof182aminoacidswith3disulfidebonds,thathasahydrophobicpocketwhichbindsretinol(vitaminA).RBPbindsretinolina1:1stoichiometry,whichservestonotonlysolubilizeretinolbutalsoprotectitfromoxidation.Wheninserum,themajorityofRBPboundwithretinolisreversIBLycomplexedwithtransthyretin(prealbumin)(2,3).Thiscomplexthentransportsretinoltospecificreceptorsofvarioustissuesinthebody.VitaminAstatusisreflectedbyserumconcentrationasitishemostaticallycontrolledanddoesnotfalluntilstoresaredramaticallyreduced(4,5).RBPhasalsobeenshowntobeausefulMarkerforrenalfunction(6)asitistotallyfilteredbytheglomeruliandreabsorbedbyproximaltubules(7).ThishasmadeurinaryRBP(uRBP)atooltostudyrenalfunctioninheart(8)orkidney(9)transplantrecipients,type1and2diabetics(10),andinpeopleexposedtouraniumfromminingoperations(11).MeasurementofuRBPlevelshasalsobeenusefulindetectionandcharacterizationofdiseasesincludinghypertension(12)andcertaincancers(13,14). |
References | 1.BlanerWS.EndocrRev.1989Aug;10(3):308–16. 2.WolfG.Physiol.Rev.1984Jul;64(3):873-937. 3.PetersenPA.J.Biol.Chem.1971,246:34-43. 4.GoodmanDS,BlanerWS.inTheRetinoids,eds.SpornMB,RobertsAB,GoodmanDS,(Orlando:AcademicPress,1984)vol.2,1-39. 5.Olson,JA.ModernNutritioninHealthandDisease,eds.ShilsME,OlsonJA,ShikeM,(Philadelphia:Lea&Febiger,1994)8thed.287–307. 6.PetersonPA,andBerggårdI.J.Biol.Chem.1971246:25-33. 7.BernardAM,andLauwerysRR.Clin.Chem.198127:1781-2. 8.CamaraNO,MatosAC,RodriguesDAetal.TheLancet2001357:856-857. 9.HosakaB,ParkSI,FelipeCR,GarciaRG,MachadoPG,PereiraAB,Tedesco-SilvaHandMedina-PestanaJO.TransplantProc.200335:1341-1343. 10.AbahusainMA,WrightJ,DickersonJWTanddeVolEB.AmJClinNutr.2004;79:218-25. 11.WyattSA,ReitzLV,CroleyTR,HawkinsD,BarrettE,McKeownA,PowellN,WestA,HamnerTandRoysterMO.JExpSciEnvironEpidem.200818:59-75. 12.BangLE,HolmJandSvendsenTL.Am.J.Hypertens19969:1024-1028. 13.MoiseAR,NoyN,PalczewskiK,BlanerWS.Biochemistry20079:1024-1028. 14.GavrilovV,YermiahuT,GorodischerR.KidneyInt.2006Jan;69(1):173-7. |
TypicalStandardCurvefortheRetinolBindingProteinUrinaryEIAKit(EnzymeImmunoassay)StressXpress®–SKT-218.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:3.906–1000ng/ml.
Linearitywasdeterminedbytakingtwohumanurinesamplesdiluted1:2,onewithalowdilutedRBPlevelof14.5ng/mLandonewithahigherdilutedlevelof299.2ng/mLandmixingthemingivenratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.
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StressMarq Biosciences Inc.位于加拿大维多利亚,是一家生物科技公司,专门从事试剂与试剂盒研究。我们有强大的国际经销商网络,主要为私人和公众客户提供经我们多次试验成功的试剂,服务范围遍及全球40多个国家。 我们公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。 通过定位好以上四个领域,研究人员能够运用研究相关的整套试剂工具,将研究产品重点放在热休克蛋白70和90(HSP70和HSP90)两个核心领域。产品主要包括ELISA试剂盒、抑制剂、蛋白质(包括无内毒素制剂,而非低内毒素制剂)以及经过高级验证的抗体,产品在全球范围内均有销售。 StressMarq因其试剂验证质量过硬,跻身为高端公司。研究人员能放心使用抗体产品,因为本产品将具有免疫印迹(WB)、免疫沉淀、组织染色技术的功能,如免疫组织化学(IHC)和免疫细胞化学(ICC)。 本公司提供氧化应激产品,如8OHdG(DNA受损)ELISA试剂盒,同时还提供专门的抗体,适用于非定量技术,如免疫组化。在免疫组化中,组织的目标可视化更为重要。除了提供用于进一步研究的相关工具外,StressMarq还提供新的尖端研究工具,用于开发新的生物项目,包括全球独一无二的α B晶体蛋白ELISA试剂盒(现在是公认的乳腺癌标志物)以及热休克同源蛋白70(Hsc70)ELISA试剂盒。本公司已将专业技术扩展到抗体、小分子离子通道与载体等更新领域,前者包括当前热门的核心领域:Nav、Cav、TRP、Kv、KCNQ、HCN,后者包括当前热门的核心领域:ENaC、NCC和NKCC2以及主要水通道蛋白载体。